Abstract

We report a lab-on-a-chip (LOC) that can separate and detect Escherichia coli (E. coli) in simulated urine samples for urinary tract infection (UTI) applications. The LOC consists of two (concentration and sensing) chambers connected in series and an integrated impedance detector. The two-chamber approach is designed to reduce the nonspecific absorption of a protein, e.g., albumin, that potentially coexists with E. coli in urine. We directly separate E. coli K-12 from cocktail urine in a concentration chamber containing microsized magnetic beads conjugated with anti-E. coli antibody. The immobilized E. coli is transferred to a sensing chamber for the impedance measurement. The measurement at the concentration chamber suffers from nonspecific absorption of albumin on the gold electrode, which may lead to false-positive response. By contrast, the measured impedance at the sensing chamber shows a ∼60-k impedance change. This is a clear distinction between 6.4 × 104 and 6.4 × 105 CFU/mL, covering the threshold of UTI (105 CFU/mL). The sensitivity of the LOC in detecting E. coli is characterized to be at least 3.4 ×104 CFU/mL.We also characterized the LOC for different age groups and white blood cell spiked samples. These preliminary data show promising potential for application in portable LOC devices for UTI detection.

Original languageEnglish (US)
Article number5968536
Pages (from-to)819-827
Number of pages9
JournalJournal of Microelectromechanical Systems
Volume20
Issue number4
DOIs
StatePublished - Aug 1 2011

Keywords

  • Escherichia coli (E. coli)
  • lab-on-a-chip (LOC)
  • point-of-care testing
  • urinary tract infection (UTI)

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Separating and detecting escherichia coli in a microfluidic channel for urinary tract infection applications'. Together they form a unique fingerprint.

  • Cite this