TY - GEN
T1 - Sensitive and selective detection of a cancer biomarker, thyroglobulin, in undiluted human serum using competitive adsorption of proteins and pattern recognition
AU - Wang, Ran
AU - Huang, Shuai
AU - Li, Jing
AU - Chae, Junseok
N1 - Publisher Copyright:
© 2012 TRF.
PY - 2012
Y1 - 2012
N2 - We report a microfluidic-based Surface Plasmon Resonance (SPR) biosensor for rapid, real-time detection of Thyroglobulin (Tg, a sensitive indicator of persistent or recurrent differentiated thyroid cancer with abnormal concentration higher than 3 ng/mL in undiluted human serum, using competitive adsorption of proteins and pattern recognition. The SPR biosensor produces unique patterns from multiple engineered sensing surfaces, and then Linear Discriminant Analysis (LDA) is used to differentiate these patterns. The microfluidic-based biosensor demonstrates the ability to detect 2 ng/mL Tg in undiluted serum with 91.7 % and 90.0 % classification confidence level for its sensitivity and selectivity, respectively.
AB - We report a microfluidic-based Surface Plasmon Resonance (SPR) biosensor for rapid, real-time detection of Thyroglobulin (Tg, a sensitive indicator of persistent or recurrent differentiated thyroid cancer with abnormal concentration higher than 3 ng/mL in undiluted human serum, using competitive adsorption of proteins and pattern recognition. The SPR biosensor produces unique patterns from multiple engineered sensing surfaces, and then Linear Discriminant Analysis (LDA) is used to differentiate these patterns. The microfluidic-based biosensor demonstrates the ability to detect 2 ng/mL Tg in undiluted serum with 91.7 % and 90.0 % classification confidence level for its sensitivity and selectivity, respectively.
UR - http://www.scopus.com/inward/record.url?scp=84944675724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944675724&partnerID=8YFLogxK
U2 - 10.31438/trf.hh2012.102
DO - 10.31438/trf.hh2012.102
M3 - Conference contribution
AN - SCOPUS:84944675724
T3 - Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop
SP - 383
EP - 384
BT - 2012 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2012
A2 - Mehregany, Mehran
A2 - Monk, David J.
PB - Transducer Research Foundation
T2 - 2012 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2012
Y2 - 3 June 2012 through 7 June 2012
ER -