Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram, Jared Q. Gerlach, Yun Xiang, Abdel Nasser Kawde, Zong Dai, Veer P. Bhavanandan, Jeffrey LaBelle, Waret Veerasai, Lokesh Joshi, Joseph Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Original languageEnglish (US)
Pages (from-to)889-891
Number of pages3
JournalAnalyst
Volume131
Issue number8
DOIs
StatePublished - 2006

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Bioassay
Glycoside Hydrolases
Biological Assay
bioassay
assay
Glycomics
Glycosides
Assays
Research
detection

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Tangkuaram, T., Gerlach, J. Q., Xiang, Y., Kawde, A. N., Dai, Z., Bhavanandan, V. P., ... Wang, J. (2006). Sensitive and rapid electrochemical bioassay of glycosidase activity. Analyst, 131(8), 889-891. https://doi.org/10.1039/b605943k

Sensitive and rapid electrochemical bioassay of glycosidase activity. / Tangkuaram, Tanin; Gerlach, Jared Q.; Xiang, Yun; Kawde, Abdel Nasser; Dai, Zong; Bhavanandan, Veer P.; LaBelle, Jeffrey; Veerasai, Waret; Joshi, Lokesh; Wang, Joseph.

In: Analyst, Vol. 131, No. 8, 2006, p. 889-891.

Research output: Contribution to journalArticle

Tangkuaram, T, Gerlach, JQ, Xiang, Y, Kawde, AN, Dai, Z, Bhavanandan, VP, LaBelle, J, Veerasai, W, Joshi, L & Wang, J 2006, 'Sensitive and rapid electrochemical bioassay of glycosidase activity', Analyst, vol. 131, no. 8, pp. 889-891. https://doi.org/10.1039/b605943k
Tangkuaram T, Gerlach JQ, Xiang Y, Kawde AN, Dai Z, Bhavanandan VP et al. Sensitive and rapid electrochemical bioassay of glycosidase activity. Analyst. 2006;131(8):889-891. https://doi.org/10.1039/b605943k
Tangkuaram, Tanin ; Gerlach, Jared Q. ; Xiang, Yun ; Kawde, Abdel Nasser ; Dai, Zong ; Bhavanandan, Veer P. ; LaBelle, Jeffrey ; Veerasai, Waret ; Joshi, Lokesh ; Wang, Joseph. / Sensitive and rapid electrochemical bioassay of glycosidase activity. In: Analyst. 2006 ; Vol. 131, No. 8. pp. 889-891.
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