Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram; Jared Q. Gerlach; Yun Xiang; Abdel Nasser Kawde; Zong Dai; Veer P. Bhavanandan; Jeffrey LaBelle; Waret Veerasai; Lokesh Joshi; Joseph Wang

doi:10.1039/b605943k

Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram, Jared Q. Gerlach, Yun Xiang, Abdel Nasser Kawde, Zong Dai, Veer P. Bhavanandan, Jeffrey LaBelle, Waret Veerasai, Lokesh Joshi, Joseph Wang

Bioengineering, Harrington Department of

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Original language	English (US)
Pages (from-to)	889-891
Number of pages	3
Journal	Analyst
Volume	131
Issue number	8
DOIs	https://doi.org/10.1039/b605943k
State	Published - 2006

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

Access to Document

10.1039/b605943k

Cite this

@article{a905886b10c241999a573c0b6e958e28,

title = "Sensitive and rapid electrochemical bioassay of glycosidase activity",

abstract = "Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.",

author = "Tanin Tangkuaram and Gerlach, {Jared Q.} and Yun Xiang and Kawde, {Abdel Nasser} and Zong Dai and Bhavanandan, {Veer P.} and Jeffrey LaBelle and Waret Veerasai and Lokesh Joshi and Joseph Wang",

year = "2006",

doi = "10.1039/b605943k",

language = "English (US)",

volume = "131",

pages = "889--891",

journal = "Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "8",

}

TY - JOUR

T1 - Sensitive and rapid electrochemical bioassay of glycosidase activity

AU - Tangkuaram, Tanin

AU - Gerlach, Jared Q.

AU - Xiang, Yun

AU - Kawde, Abdel Nasser

AU - Dai, Zong

AU - Bhavanandan, Veer P.

AU - LaBelle, Jeffrey

AU - Veerasai, Waret

AU - Joshi, Lokesh

AU - Wang, Joseph

PY - 2006

Y1 - 2006

N2 - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

AB - Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

UR - http://www.scopus.com/inward/record.url?scp=33746437088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746437088&partnerID=8YFLogxK

U2 - 10.1039/b605943k

DO - 10.1039/b605943k

M3 - Article

C2 - 17028720

AN - SCOPUS:33746437088

SN - 0003-2654

VL - 131

SP - 889

EP - 891

JO - Analyst

JF - Analyst

IS - 8

ER -

Sensitive and rapid electrochemical bioassay of glycosidase activity

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this