Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram, Jared Q. Gerlach, Yun Xiang, Abdel Nasser Kawde, Zong Dai, Veer P. Bhavanandan, Jeffrey LaBelle, Waret Veerasai, Lokesh Joshi, Joseph Wang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Original languageEnglish (US)
Pages (from-to)889-891
Number of pages3
JournalAnalyst
Volume131
Issue number8
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Tangkuaram, T., Gerlach, J. Q., Xiang, Y., Kawde, A. N., Dai, Z., Bhavanandan, V. P., LaBelle, J., Veerasai, W., Joshi, L., & Wang, J. (2006). Sensitive and rapid electrochemical bioassay of glycosidase activity. Analyst, 131(8), 889-891. https://doi.org/10.1039/b605943k