TY - CHAP
T1 - Selfishness in Moderation
T2 - Evolutionary Success of the Yeast Plasmid
AU - Velmurugan, Soundarapandian
AU - Mehta, Shwetal
AU - Jayaram, Makkuni
N1 - Funding Information:
Work in the Jayaram laboratory on the recombination and partitioning systems of the yeast plasmid has been supported over the years by funds from the National Institutes of Health, the National Science Foundation, the Robert F. Welch Foundation, the Council for Tobacco Research, the Texas Higher Education Coordinating Board, and the Human Frontiers in Science Program.
PY - 2003
Y1 - 2003
N2 - The yeast plasmid 2-μm circle is an extrachromosomal selfish DNA element whose genetic endowments are devoted to its stable, high copy propagation. The mean steady state plasmid copy number of approximately 60 per cell appears to be evolutionarily optimized at its permissible maximum value. A plasmid-encoded negative regulatory mechanism prevents a rise in copy number that might imperil normal host metabolism and thus indirectly reduce plasmid fitness. The plasmid utilizes the host replication machinery for its own duplication. A plasmid-encoded partitioning system mediates even distribution of the replicated molecules to daughter cells, apparently by feeding into the chromosome segregation pathway. The plasmid also harbors an amplification system as a potential safeguard against a fall in copy number due to an occasional missegregation event. The 2-μm circle provides a model for how moderation of selfishness can ensure the successful persistence of an extrachromosomal element without compromising the fitness of its host.
AB - The yeast plasmid 2-μm circle is an extrachromosomal selfish DNA element whose genetic endowments are devoted to its stable, high copy propagation. The mean steady state plasmid copy number of approximately 60 per cell appears to be evolutionarily optimized at its permissible maximum value. A plasmid-encoded negative regulatory mechanism prevents a rise in copy number that might imperil normal host metabolism and thus indirectly reduce plasmid fitness. The plasmid utilizes the host replication machinery for its own duplication. A plasmid-encoded partitioning system mediates even distribution of the replicated molecules to daughter cells, apparently by feeding into the chromosome segregation pathway. The plasmid also harbors an amplification system as a potential safeguard against a fall in copy number due to an occasional missegregation event. The 2-μm circle provides a model for how moderation of selfishness can ensure the successful persistence of an extrachromosomal element without compromising the fitness of its host.
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U2 - 10.1016/S0070-2153(03)01005-6
DO - 10.1016/S0070-2153(03)01005-6
M3 - Chapter
C2 - 14584724
AN - SCOPUS:0141517795
SN - 0121531562
SN - 9780121531560
T3 - Current Topics in Developmental Biology
SP - 1
EP - 24
BT - Current Topics in Developmental Biology
PB - Academic Press Inc
ER -