Self-Assembled Signaling Aptamer Arrays for Protein Detection

Yan Liu (Inventor), Hao Yan (Inventor)

Research output: Patent

Abstract

The present invention describes a novel approach for a DNA tile directed self-assembly of signaling aptamers into high-density nanoarrays for detection of protein molecules. signaling aptamers are created by replacing one nucleotide close to the protein-binding site in the aptamer sequence with a fluorescent nucleotide analog, which generates significant increase in fluorescence upon binding to thrombin. The signaling aptamer was linked to a DNA tile designed to self-assemble into 2-dimensional DNA arrays with periodic spacing between neighboring aptamers. The high local density of the signaling aptamers displayed on the self-assembled DNA arrays allowed us to detect low concentration of protein bound to the arrays using confocal fluorescence microscope imaging.
Original languageEnglish (US)
StatePublished - May 29 2006

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DNA
Tile
Proteins
Nucleotides
Fluorescence
Patents and inventions
Thrombin
Self assembly
Microscopes
Binding Sites
Imaging techniques
Molecules
Protein Binding

Cite this

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abstract = "The present invention describes a novel approach for a DNA tile directed self-assembly of signaling aptamers into high-density nanoarrays for detection of protein molecules. signaling aptamers are created by replacing one nucleotide close to the protein-binding site in the aptamer sequence with a fluorescent nucleotide analog, which generates significant increase in fluorescence upon binding to thrombin. The signaling aptamer was linked to a DNA tile designed to self-assemble into 2-dimensional DNA arrays with periodic spacing between neighboring aptamers. The high local density of the signaling aptamers displayed on the self-assembled DNA arrays allowed us to detect low concentration of protein bound to the arrays using confocal fluorescence microscope imaging.",
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AB - The present invention describes a novel approach for a DNA tile directed self-assembly of signaling aptamers into high-density nanoarrays for detection of protein molecules. signaling aptamers are created by replacing one nucleotide close to the protein-binding site in the aptamer sequence with a fluorescent nucleotide analog, which generates significant increase in fluorescence upon binding to thrombin. The signaling aptamer was linked to a DNA tile designed to self-assemble into 2-dimensional DNA arrays with periodic spacing between neighboring aptamers. The high local density of the signaling aptamers displayed on the self-assembled DNA arrays allowed us to detect low concentration of protein bound to the arrays using confocal fluorescence microscope imaging.

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