A method for assembling and reading a DNA hybridization array of nm-scale dimensions. A unique DNA sequence is attached at each vertex of a self-assembled array of NDA tiles. Choice of the correct sequence for assembly of the array results in a known probe sequence at known locations on the self-assembled array. Hybridization with targe DNA can be carried out with the array suspended in solution. The array is ready by depositing it onto a flat surface and reading out the sites of hybridization with an atomic force microscope. The advantages of this invention are:- Density: each targe molecule occupies an area of only 20x20nm, yielding a possible density of 2.5x10^8 sequences in 1 square cm.- Ease and low unit cost of fabrication: A nM-scale synthesis yields 10^14 copies of the array- Use in-situ: The targe sequences are attachted to a solubale nanostructure, so no surface chemistry is needed, opening up new possibilities for biochemical operations on the array- Minute reagent requirements: the array is capable of operating at the single molecule level.
|Original language||English (US)|
|State||Published - Jan 13 2005|