Selenate and Nitrate Bioreductions Using Methane as the Electron Donor in a Membrane Biofilm Reactor

Chun Yu Lai; Li Lian Wen; Ling Dong Shi; Kan Kan Zhao; Yi Qi Wang; Xiaoe Yang; Bruce Rittmann; Chen Zhou; Youneng Tang; Ping Zheng; He Ping Zhao

doi:10.1021/acs.est.6b02807

Selenate and Nitrate Bioreductions Using Methane as the Electron Donor in a Membrane Biofilm Reactor

Chun Yu Lai, Li Lian Wen, Ling Dong Shi, Kan Kan Zhao, Yi Qi Wang, Xiaoe Yang, Bruce Rittmann, Chen Zhou, Youneng Tang, Ping Zheng, He Ping Zhao

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111 Scopus citations

Abstract

Selenate (SeO₄^2-) bioreduction is possible with oxidation of a range of organic or inorganic electron donors, but it never has been reported with methane gas (CH₄) as the electron donor. In this study, we achieved complete SeO₄^2- bioreduction in a membrane biofilm reactor (MBfR) using CH₄ as the sole added electron donor. The introduction of nitrate (NO₃^-) slightly inhibited SeO₄^2- reduction, but the two oxyanions were simultaneously reduced, even when the supply rate of CH₄ was limited. The main SeO₄^2--reduction product was nanospherical Se⁰, which was identified by scanning electron microscopy coupled to energy dispersive X-ray analysis (SEM-EDS). Community analysis provided evidence for two mechanisms for SeO₄^2- bioreduction in the CH₄-based MBfR: a single methanotrophic genus, such as Methylomonas, performed CH₄ oxidation directly coupled to SeO₄^2- reduction, and a methanotroph oxidized CH₄ to form organic metabolites that were electron donors for a synergistic SeO₄^2--reducing bacterium.

Original language	English (US)
Pages (from-to)	10179-10186
Number of pages	8
Journal	Environmental Science and Technology
Volume	50
Issue number	18
DOIs	https://doi.org/10.1021/acs.est.6b02807
State	Published - Sep 20 2016

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry

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10.1021/acs.est.6b02807

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title = "Selenate and Nitrate Bioreductions Using Methane as the Electron Donor in a Membrane Biofilm Reactor",

abstract = "Selenate (SeO42-) bioreduction is possible with oxidation of a range of organic or inorganic electron donors, but it never has been reported with methane gas (CH4) as the electron donor. In this study, we achieved complete SeO42- bioreduction in a membrane biofilm reactor (MBfR) using CH4 as the sole added electron donor. The introduction of nitrate (NO3-) slightly inhibited SeO42- reduction, but the two oxyanions were simultaneously reduced, even when the supply rate of CH4 was limited. The main SeO42--reduction product was nanospherical Se0, which was identified by scanning electron microscopy coupled to energy dispersive X-ray analysis (SEM-EDS). Community analysis provided evidence for two mechanisms for SeO42- bioreduction in the CH4-based MBfR: a single methanotrophic genus, such as Methylomonas, performed CH4 oxidation directly coupled to SeO42- reduction, and a methanotroph oxidized CH4 to form organic metabolites that were electron donors for a synergistic SeO42--reducing bacterium.",

author = "Lai, {Chun Yu} and Wen, {Li Lian} and Shi, {Ling Dong} and Zhao, {Kan Kan} and Wang, {Yi Qi} and Xiaoe Yang and Bruce Rittmann and Chen Zhou and Youneng Tang and Ping Zheng and Zhao, {He Ping}",

note = "Funding Information: We greatly thank the National Natural Science Foundation of China (Grant No. 21377109, 21577123), the Fundamental Research Funds for the Central Universities (2016QNA6007), and Public Welfare Project of the Science and Technology Department of Zhejiang Province (2015C33016) for their financial support. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = sep,

day = "20",

doi = "10.1021/acs.est.6b02807",

language = "English (US)",

volume = "50",

pages = "10179--10186",

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TY - JOUR

T1 - Selenate and Nitrate Bioreductions Using Methane as the Electron Donor in a Membrane Biofilm Reactor

AU - Lai, Chun Yu

AU - Wen, Li Lian

AU - Shi, Ling Dong

AU - Zhao, Kan Kan

AU - Wang, Yi Qi

AU - Yang, Xiaoe

AU - Rittmann, Bruce

AU - Zhou, Chen

AU - Tang, Youneng

AU - Zheng, Ping

AU - Zhao, He Ping

N1 - Funding Information: We greatly thank the National Natural Science Foundation of China (Grant No. 21377109, 21577123), the Fundamental Research Funds for the Central Universities (2016QNA6007), and Public Welfare Project of the Science and Technology Department of Zhejiang Province (2015C33016) for their financial support. Publisher Copyright: © 2016 American Chemical Society.

PY - 2016/9/20

Y1 - 2016/9/20

N2 - Selenate (SeO42-) bioreduction is possible with oxidation of a range of organic or inorganic electron donors, but it never has been reported with methane gas (CH4) as the electron donor. In this study, we achieved complete SeO42- bioreduction in a membrane biofilm reactor (MBfR) using CH4 as the sole added electron donor. The introduction of nitrate (NO3-) slightly inhibited SeO42- reduction, but the two oxyanions were simultaneously reduced, even when the supply rate of CH4 was limited. The main SeO42--reduction product was nanospherical Se0, which was identified by scanning electron microscopy coupled to energy dispersive X-ray analysis (SEM-EDS). Community analysis provided evidence for two mechanisms for SeO42- bioreduction in the CH4-based MBfR: a single methanotrophic genus, such as Methylomonas, performed CH4 oxidation directly coupled to SeO42- reduction, and a methanotroph oxidized CH4 to form organic metabolites that were electron donors for a synergistic SeO42--reducing bacterium.

AB - Selenate (SeO42-) bioreduction is possible with oxidation of a range of organic or inorganic electron donors, but it never has been reported with methane gas (CH4) as the electron donor. In this study, we achieved complete SeO42- bioreduction in a membrane biofilm reactor (MBfR) using CH4 as the sole added electron donor. The introduction of nitrate (NO3-) slightly inhibited SeO42- reduction, but the two oxyanions were simultaneously reduced, even when the supply rate of CH4 was limited. The main SeO42--reduction product was nanospherical Se0, which was identified by scanning electron microscopy coupled to energy dispersive X-ray analysis (SEM-EDS). Community analysis provided evidence for two mechanisms for SeO42- bioreduction in the CH4-based MBfR: a single methanotrophic genus, such as Methylomonas, performed CH4 oxidation directly coupled to SeO42- reduction, and a methanotroph oxidized CH4 to form organic metabolites that were electron donors for a synergistic SeO42--reducing bacterium.

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Selenate and Nitrate Bioreductions Using Methane as the Electron Donor in a Membrane Biofilm Reactor

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