TY - JOUR
T1 - Selective targeting of unipolar brush cell subtypes by cerebellar mossy fibers
AU - Balmer, Timothy S.
AU - Trussell, Laurence O.
N1 - Funding Information:
We would like to thank Sacha Nelson and Adam Hantman for the TCGO mice, Adam Hantman and Kim Ritola for the retro-AAVs, Jocelyn Krey for help with inner ear dissection, Ruby Larisch and Jen-nifer Goldsmith for help with mouse husbandry, Peter Barr-Gillespie for the Myo7A antibody, Aurelie Snyder, Stephanie Kaech Petrie and Crystal Chaw for help with microscopy, and NIH F32 DC014878, NIH K99 DC016905, and Hearing Health Foundation Emerging Research Grant to TSB, NIH R01 NS028901 and DC004450 to LOT, NIH P30 NS0618000 to S Aicher.
Publisher Copyright:
© Lee et al.
PY - 2019/4
Y1 - 2019/4
N2 - In vestibular cerebellum, primary afferents carry signals from single vestibular end organs, whereas secondary afferents from vestibular nucleus carry integrated signals. Selective targeting of distinct mossy fibers determines how the cerebellum processes vestibular signals. We focused on vestibular projections to ON and OFF classes of unipolar brush cells (UBCs), which transform single mossy fiber signals into long-lasting excitation or inhibition respectively, and impact the activity of ensembles of granule cells. To determine whether these contacts are indeed selective, connectivity was traced back from UBC to specific ganglion cell, hair cell and vestibular organ subtypes in mice. We show that a specialized subset of primary afferents contacts ON UBCs, but not OFF UBCs, while secondary afferents contact both subtypes. Striking anatomical differences were observed between primary and secondary afferents, their synapses, and the UBCs they contact. Thus, each class of UBC functions to transform specific signals through distinct anatomical pathways.
AB - In vestibular cerebellum, primary afferents carry signals from single vestibular end organs, whereas secondary afferents from vestibular nucleus carry integrated signals. Selective targeting of distinct mossy fibers determines how the cerebellum processes vestibular signals. We focused on vestibular projections to ON and OFF classes of unipolar brush cells (UBCs), which transform single mossy fiber signals into long-lasting excitation or inhibition respectively, and impact the activity of ensembles of granule cells. To determine whether these contacts are indeed selective, connectivity was traced back from UBC to specific ganglion cell, hair cell and vestibular organ subtypes in mice. We show that a specialized subset of primary afferents contacts ON UBCs, but not OFF UBCs, while secondary afferents contact both subtypes. Striking anatomical differences were observed between primary and secondary afferents, their synapses, and the UBCs they contact. Thus, each class of UBC functions to transform specific signals through distinct anatomical pathways.
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U2 - 10.7554/eLife.44964
DO - 10.7554/eLife.44964
M3 - Article
C2 - 30994458
AN - SCOPUS:85064973366
VL - 8
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e44964
ER -