Abstract

Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all the microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell-culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture.

Original languageEnglish (US)
Article number7322185
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume22
Issue number3
DOIs
StatePublished - May 1 2016

Fingerprint

ultrashort pulsed lasers
Disinfection
Pulsed lasers
Cell culture
Photonics
photonics
viruses
microorganisms
Viruses
Biological materials preservation
Drug products
bacteria
Bacteria
contamination
Contamination
biotechnology
pathogens
fungi
yeast
Pathogens

Keywords

  • cell cultures
  • pathogens
  • Photonic disinfection
  • ultrashort pulsed laser

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

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title = "Selective Photonic Disinfection of Cell Culture Using a Visible Ultrashort Pulsed Laser",
abstract = "Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all the microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell-culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture.",
keywords = "cell cultures, pathogens, Photonic disinfection, ultrashort pulsed laser",
author = "Tsen, {Shaw Wei D} and Karen Kibler and Bertram Jacobs and Fay, {Justin C.} and Nataly Podolnikova and Tatiana Ugarova and Samuel Achilefu and Kong-Thon Tsen",
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AU - Tsen, Shaw Wei D

AU - Kibler, Karen

AU - Jacobs, Bertram

AU - Fay, Justin C.

AU - Podolnikova, Nataly

AU - Ugarova, Tatiana

AU - Achilefu, Samuel

AU - Tsen, Kong-Thon

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all the microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell-culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture.

AB - Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all the microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell-culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture.

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KW - Photonic disinfection

KW - ultrashort pulsed laser

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