Abstract
Recently, femtosecond laser technology has been shown to be effective in the inactivation of non-pathogenic viruses. In this paper, we demonstrate for the first time that infectious numbers of pathogenic viruses such as Human Immunodeficiency Virus (HIV) can be reduced by irradiation with subpicosecond near infrared laser pulses at a moderate laser power density. By comparing the threshold laser power density for the inactivation of HIV with those of human red blood cells and mouse dendritic cells, we conclude that it is plausible to use the ultrashort pulsed laser to selectively inactivate blood-borne pathogens such as HIV while leaving the sensitive materials like human red blood cells unharmed. This finding has important implications in the development of a new laser technology for disinfection of viral pathogens in blood products and in the clinic.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 7175 |
| DOIs | |
| State | Published - 2009 |
| Event | Optical Interactions with Tissue and Cells XX - San Jose, CA, United States Duration: Jan 26 2009 → Jan 28 2009 |
Other
| Other | Optical Interactions with Tissue and Cells XX |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/26/09 → 1/28/09 |
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Keywords
- Human Immunodeficiency Virus
- Pathogen inactivation
- Ultrashort pulsed laser
- Viral load reduction
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Selective inactivation of Human Immunodeficiency Virus with an ultrashort pulsed laser. / Tsen, Kong-Thon; Tsen, Shaw Wei D; Hung, Chien Fu; Wu, T. C.; Kibler, Karen; Jacobs, Bertram; Kiang, Juliann G.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7175 2009. 717510.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Selective inactivation of Human Immunodeficiency Virus with an ultrashort pulsed laser
AU - Tsen, Kong-Thon
AU - Tsen, Shaw Wei D
AU - Hung, Chien Fu
AU - Wu, T. C.
AU - Kibler, Karen
AU - Jacobs, Bertram
AU - Kiang, Juliann G.
PY - 2009
Y1 - 2009
N2 - Recently, femtosecond laser technology has been shown to be effective in the inactivation of non-pathogenic viruses. In this paper, we demonstrate for the first time that infectious numbers of pathogenic viruses such as Human Immunodeficiency Virus (HIV) can be reduced by irradiation with subpicosecond near infrared laser pulses at a moderate laser power density. By comparing the threshold laser power density for the inactivation of HIV with those of human red blood cells and mouse dendritic cells, we conclude that it is plausible to use the ultrashort pulsed laser to selectively inactivate blood-borne pathogens such as HIV while leaving the sensitive materials like human red blood cells unharmed. This finding has important implications in the development of a new laser technology for disinfection of viral pathogens in blood products and in the clinic.
AB - Recently, femtosecond laser technology has been shown to be effective in the inactivation of non-pathogenic viruses. In this paper, we demonstrate for the first time that infectious numbers of pathogenic viruses such as Human Immunodeficiency Virus (HIV) can be reduced by irradiation with subpicosecond near infrared laser pulses at a moderate laser power density. By comparing the threshold laser power density for the inactivation of HIV with those of human red blood cells and mouse dendritic cells, we conclude that it is plausible to use the ultrashort pulsed laser to selectively inactivate blood-borne pathogens such as HIV while leaving the sensitive materials like human red blood cells unharmed. This finding has important implications in the development of a new laser technology for disinfection of viral pathogens in blood products and in the clinic.
KW - Human Immunodeficiency Virus
KW - Pathogen inactivation
KW - Ultrashort pulsed laser
KW - Viral load reduction
UR - http://www.scopus.com/inward/record.url?scp=65349101764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349101764&partnerID=8YFLogxK
U2 - 10.1117/12.808532
DO - 10.1117/12.808532
M3 - Conference contribution
AN - SCOPUS:65349101764
VL - 7175
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -