Selective chemical inactivation of AAA proteins reveals distinct functions of proteasomal ATPases

Steven Jon Russell, Fernando Gonzalez, Leemor Joshua-Tor, Stephen Johnston

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The 26S proteasome contains six highly related ATPases of the AAA family. We have developed a strategy that allows selective inhibition of individual proteasomal ATPases in the intact proteasome. Mutation of a threonine in the active site of Sug1/Rpt6 or Sug2/Rpt4 to a cysteine sensitizes these proteins to inactivation through alkylation by the sulfhydryl modifying agent NEM. Using this technique the individual contributions of Sug1 and Sug2 to proteasome function can be assessed. Results: We show that both Sug1 and Sug2 can be selectively alkylated by NEM in the context of the intact 26S complex and as predicted by structural modeling, this inactivates the ATPase function. Using this technique we demonstrate that both Sug 1 and 2 are required for full peptidase activity of the proteasome and that their functions are not redundant. Kinetic analysis suggests that Sug2 may have an important role in maintaining the interaction between the 19S regulatory complex and the 20S proteasome. In contrast, inhibition of Sug1 apparently decreases peptidase activity of the 26S proteasome by another mechanism. Conclusions: These results describe a useful technique for the selective inactivation of AAA proteins. In addition, they also demonstrate that the functions of two related proteasomal AAA proteins are not redundant, suggesting differential roles of proteasomal AAA proteins in protein degradation.

Original languageEnglish (US)
Pages (from-to)941-950
Number of pages10
JournalChemistry and Biology
Volume8
Issue number10
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Adenosine Triphosphatases
Proteins
Peptide Hydrolases
Alkylation
Threonine
Proteolysis
Cysteine
Catalytic Domain
Mutation
Degradation
Kinetics
ATP dependent 26S protease

Keywords

  • AAA proteins
  • Chemical inactivation
  • Proteasomal ATPases

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Selective chemical inactivation of AAA proteins reveals distinct functions of proteasomal ATPases. / Russell, Steven Jon; Gonzalez, Fernando; Joshua-Tor, Leemor; Johnston, Stephen.

In: Chemistry and Biology, Vol. 8, No. 10, 2001, p. 941-950.

Research output: Contribution to journalArticle

Russell, Steven Jon ; Gonzalez, Fernando ; Joshua-Tor, Leemor ; Johnston, Stephen. / Selective chemical inactivation of AAA proteins reveals distinct functions of proteasomal ATPases. In: Chemistry and Biology. 2001 ; Vol. 8, No. 10. pp. 941-950.
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