Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P

Yan Yuan, Sidney Altman

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Any RNA, when in a complex with another oligoribonucleotide known as an external guide sequence (EGS), can become a substrate for ribonuclease P. Simulation of evolution in vitro was used to select EGSs that bind tightly to a target substrate messenger RNA and that increase the efficiency of cleavage of the target by human ribonuclease P to a level equal to that achieved with natural substrates. The most efficient EGSs form transfer RNA precursor-like structures with the target RNA, in which the analog of the anticodon stem has been disrupted, an indication that selection for the optimal substrate for ribonuclease P yields an RNA structure different from that of present-day transfer RNA precursors.

Original languageEnglish (US)
Pages (from-to)1269-1273
Number of pages5
JournalScience
Volume263
Issue number5151
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • General

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