Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

A. Frankel, D. Schlossman, P. Welsh, A. Hertler, D. Withers, S. Johnston

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Abstract

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving residues located in the proposed active-site cleft.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalMolecular and cellular biology
Volume9
Issue number2
DOIs
StatePublished - Feb 1989

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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