Secondary structure adopted by the Gly-Gly-X repetitive regions of dragline spider silk

Geoffrey M. Gray, Arjan van der Vaart, Chengchen Guo, Justin Jones, David Onofrei, Brian Cherry, Randolph V. Lewis, Jeffery Yarger, Gregory P. Holland

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Solid-state NMR and molecular dynamics (MD) simulations are presented to help elucidate the molecular secondary structure of poly(Gly-Gly-X), which is one of the most common structural repetitive motifs found in orb-weaving dragline spider silk proteins. The combination of NMR and computational experiments provides insight into the molecular secondary structure of poly(Gly-Gly-X) segments and provides further support that these regions are disordered and primarily non- β-sheet. Furthermore, the combination of NMR and MD simulations illustrate the possibility for several secondary structural elements in the poly(Gly-Gly-X) regions of dragline silks, including β-turns, 310-helicies, and coil structures with a negligible population of β-helix observed.

Original languageEnglish (US)
Article number2023
JournalInternational Journal of Molecular Sciences
Volume17
Issue number12
DOIs
StatePublished - Dec 2 2016

Fingerprint

spiders
silk
Spiders
Silk
Nucleic Acid Repetitive Sequences
Molecular Dynamics Simulation
Molecular Structure
Nuclear magnetic resonance
nuclear magnetic resonance
Molecular dynamics
weaving
molecular dynamics
Computer simulation
helices
coils
simulation
Population
solid state
proteins
Proteins

Keywords

  • Molecular dynamics
  • NMR
  • Secondary structure
  • Solid-state NMR
  • Spider silk

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Gray, G. M., van der Vaart, A., Guo, C., Jones, J., Onofrei, D., Cherry, B., ... Holland, G. P. (2016). Secondary structure adopted by the Gly-Gly-X repetitive regions of dragline spider silk. International Journal of Molecular Sciences, 17(12), [2023]. https://doi.org/10.3390/ijms17122023

Secondary structure adopted by the Gly-Gly-X repetitive regions of dragline spider silk. / Gray, Geoffrey M.; van der Vaart, Arjan; Guo, Chengchen; Jones, Justin; Onofrei, David; Cherry, Brian; Lewis, Randolph V.; Yarger, Jeffery; Holland, Gregory P.

In: International Journal of Molecular Sciences, Vol. 17, No. 12, 2023, 02.12.2016.

Research output: Contribution to journalArticle

Gray, GM, van der Vaart, A, Guo, C, Jones, J, Onofrei, D, Cherry, B, Lewis, RV, Yarger, J & Holland, GP 2016, 'Secondary structure adopted by the Gly-Gly-X repetitive regions of dragline spider silk', International Journal of Molecular Sciences, vol. 17, no. 12, 2023. https://doi.org/10.3390/ijms17122023
Gray, Geoffrey M. ; van der Vaart, Arjan ; Guo, Chengchen ; Jones, Justin ; Onofrei, David ; Cherry, Brian ; Lewis, Randolph V. ; Yarger, Jeffery ; Holland, Gregory P. / Secondary structure adopted by the Gly-Gly-X repetitive regions of dragline spider silk. In: International Journal of Molecular Sciences. 2016 ; Vol. 17, No. 12.
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