Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors

Xiao TAN, Fanxiang KONG, Qingfei ZENG, Huansheng Cao, Shanqin QIAN, Min ZHANG

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In order to monitor the changes of Microcystis along with temporal and spatial variations, seasonal variation of Microcystis in Lake Taihu was investigated by 16S-23S rRNA internal transcribed spacer denaturing gradient gel electrophoresis (16S-23S rRNA-ITS DGGE) and microscopic evaluation. Samples were collected quarterly at four sites (River Mouth, Meiliang Bay, Cross Area, and Lake Center) from August 2006 to April 2007. Results showed that Microcystis dominated total phytoplankton abundance at the four sites in all seasons except winter. The average annual abundance of Microcystis was relatively high at River Mouth and Meiliang Bay, reaching 81.22 × 10 6 and 61.32 × 106 cells/L, respectively. For temporal variations, Shannon-Wiener diversity index (H′) according to DGGE profile revealed the richness of Microcystis in summer (H′ = 1.375 ± 0.034) and winter (H′ = 1.650 ± 0.032) was lower than that in spring (H′ = 2.078 ± 0.031) and autumn (H′ = 2.365 ± 0.032) (P < 0.05). While for spatial variations, the richness of Microcystis at River Mouth (H′ = 2.015 ± 0.074) was higher than at other sites during four seasons (P < 0.01). Very few differences of Microcystis diversity in the same season were observed among the other three sites (P > 0.05). Canonical correspondence analysis (CCA) was performed to elucidate the relationships between Microcystis operational taxonomic units (OTUs) composition and the environmental factors. Results of CCA revealed that temperature was strongly positively correlated with the first axis (r = 0.963), while TSS was negative correlated with the second axis (r = -0.716). Phylogenetic tree based on the sequencing results of target bands on DGGE gel indicated that samples collected in summer and winter constituted two separated clusters.

Original languageEnglish (US)
Pages (from-to)892-899
Number of pages8
JournalJournal of Environmental Sciences
Volume21
Issue number7
DOIs
StatePublished - Jul 20 2009
Externally publishedYes

Fingerprint

Lakes
environmental factor
Gels
seasonal variation
Rivers
correspondence analysis
Phytoplankton
winter
lake
temporal variation
gel
Electrophoresis
summer
diversity index
river
electrokinesis
spatial variation
Chemical analysis
autumn
phytoplankton

Keywords

  • blooms
  • canonical correspondence analysis
  • DGGE
  • Lake Taihu
  • Microcystis
  • rRNA-ITS

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors. / TAN, Xiao; KONG, Fanxiang; ZENG, Qingfei; Cao, Huansheng; QIAN, Shanqin; ZHANG, Min.

In: Journal of Environmental Sciences, Vol. 21, No. 7, 20.07.2009, p. 892-899.

Research output: Contribution to journalArticle

TAN, Xiao ; KONG, Fanxiang ; ZENG, Qingfei ; Cao, Huansheng ; QIAN, Shanqin ; ZHANG, Min. / Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors. In: Journal of Environmental Sciences. 2009 ; Vol. 21, No. 7. pp. 892-899.
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abstract = "In order to monitor the changes of Microcystis along with temporal and spatial variations, seasonal variation of Microcystis in Lake Taihu was investigated by 16S-23S rRNA internal transcribed spacer denaturing gradient gel electrophoresis (16S-23S rRNA-ITS DGGE) and microscopic evaluation. Samples were collected quarterly at four sites (River Mouth, Meiliang Bay, Cross Area, and Lake Center) from August 2006 to April 2007. Results showed that Microcystis dominated total phytoplankton abundance at the four sites in all seasons except winter. The average annual abundance of Microcystis was relatively high at River Mouth and Meiliang Bay, reaching 81.22 × 10 6 and 61.32 × 106 cells/L, respectively. For temporal variations, Shannon-Wiener diversity index (H′) according to DGGE profile revealed the richness of Microcystis in summer (H′ = 1.375 ± 0.034) and winter (H′ = 1.650 ± 0.032) was lower than that in spring (H′ = 2.078 ± 0.031) and autumn (H′ = 2.365 ± 0.032) (P < 0.05). While for spatial variations, the richness of Microcystis at River Mouth (H′ = 2.015 ± 0.074) was higher than at other sites during four seasons (P < 0.01). Very few differences of Microcystis diversity in the same season were observed among the other three sites (P > 0.05). Canonical correspondence analysis (CCA) was performed to elucidate the relationships between Microcystis operational taxonomic units (OTUs) composition and the environmental factors. Results of CCA revealed that temperature was strongly positively correlated with the first axis (r = 0.963), while TSS was negative correlated with the second axis (r = -0.716). Phylogenetic tree based on the sequencing results of target bands on DGGE gel indicated that samples collected in summer and winter constituted two separated clusters.",
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