Search for Peptides Specifically Binding with the B7-2 Costimulatory Molecule

E. A. Kolosova; O. E. Viktorina; A. I. Chapoval; D. N. Shcherbakov

doi:10.1134/S106816202106011X

Search for Peptides Specifically Binding with the B7-2 Costimulatory Molecule

E. A. Kolosova, O. E. Viktorina, A. I. Chapoval, D. N. Shcherbakov

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract—: The interaction of B7-1/B7-2 ligands with CD28/CTLA-4 receptors plays a key role in the regulation of the immune response. The aim of this study was to find and study peptides that interact with the human B7-2 molecule. In the course of the work, three rounds of affinity selection were carried out and individual phage clones were selected, which include peptides with varying degrees of interaction with the coregulatory target B7-2. As a result of DNA sequencing of selected phages, nucleotide sequences encoding peptides that specifically bind to B7-2 were obtained. The identified peptides can be used as a basis for the development of immunotherapeutic drugs for regulating the immune response in the treatment of oncological diseases.

Original language	English (US)
Pages (from-to)	1220-1224
Number of pages	5
Journal	Russian Journal of Bioorganic Chemistry
Volume	47
Issue number	6
DOIs	https://doi.org/10.1134/S106816202106011X
State	Published - Nov 2021
Externally published	Yes

Keywords

CD86
family B7
immunotherapy
oncological diseases
phage display

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1134/S106816202106011X

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title = "Search for Peptides Specifically Binding with the B7-2 Costimulatory Molecule",

abstract = "Abstract—: The interaction of B7-1/B7-2 ligands with CD28/CTLA-4 receptors plays a key role in the regulation of the immune response. The aim of this study was to find and study peptides that interact with the human B7-2 molecule. In the course of the work, three rounds of affinity selection were carried out and individual phage clones were selected, which include peptides with varying degrees of interaction with the coregulatory target B7-2. As a result of DNA sequencing of selected phages, nucleotide sequences encoding peptides that specifically bind to B7-2 were obtained. The identified peptides can be used as a basis for the development of immunotherapeutic drugs for regulating the immune response in the treatment of oncological diseases.",

keywords = "CD86, family B7, immunotherapy, oncological diseases, phage display",

author = "Kolosova, {E. A.} and Viktorina, {O. E.} and Chapoval, {A. I.} and Shcherbakov, {D. N.}",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research (grants no. 19-44-220008, 17-54-33003) and the state assignment of the Ministry of Education and Science of Russia (no. FZMW-2020-0007). Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = nov,

doi = "10.1134/S106816202106011X",

language = "English (US)",

volume = "47",

pages = "1220--1224",

journal = "Russian Journal of Bioorganic Chemistry",

issn = "1068-1620",

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number = "6",

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T1 - Search for Peptides Specifically Binding with the B7-2 Costimulatory Molecule

AU - Kolosova, E. A.

AU - Viktorina, O. E.

AU - Chapoval, A. I.

AU - Shcherbakov, D. N.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (grants no. 19-44-220008, 17-54-33003) and the state assignment of the Ministry of Education and Science of Russia (no. FZMW-2020-0007). Publisher Copyright: © 2021, The Author(s).

PY - 2021/11

Y1 - 2021/11

N2 - Abstract—: The interaction of B7-1/B7-2 ligands with CD28/CTLA-4 receptors plays a key role in the regulation of the immune response. The aim of this study was to find and study peptides that interact with the human B7-2 molecule. In the course of the work, three rounds of affinity selection were carried out and individual phage clones were selected, which include peptides with varying degrees of interaction with the coregulatory target B7-2. As a result of DNA sequencing of selected phages, nucleotide sequences encoding peptides that specifically bind to B7-2 were obtained. The identified peptides can be used as a basis for the development of immunotherapeutic drugs for regulating the immune response in the treatment of oncological diseases.

AB - Abstract—: The interaction of B7-1/B7-2 ligands with CD28/CTLA-4 receptors plays a key role in the regulation of the immune response. The aim of this study was to find and study peptides that interact with the human B7-2 molecule. In the course of the work, three rounds of affinity selection were carried out and individual phage clones were selected, which include peptides with varying degrees of interaction with the coregulatory target B7-2. As a result of DNA sequencing of selected phages, nucleotide sequences encoding peptides that specifically bind to B7-2 were obtained. The identified peptides can be used as a basis for the development of immunotherapeutic drugs for regulating the immune response in the treatment of oncological diseases.

KW - CD86

KW - family B7

KW - immunotherapy

KW - oncological diseases

KW - phage display

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U2 - 10.1134/S106816202106011X

DO - 10.1134/S106816202106011X

M3 - Article

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JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

IS - 6

ER -

Search for Peptides Specifically Binding with the B7-2 Costimulatory Molecule

Abstract

Keywords

ASJC Scopus subject areas

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