TY - JOUR
T1 - Screening the whole genome of a pathogen in vivo for individual protective antigens
AU - Stemke-Hale, Katherine
AU - Kaltenboeck, Bernhard
AU - DeGraves, Fred J.
AU - Sykes, Kathryn F.
AU - Huang, Jin
AU - Bu, Chun Hui
AU - Johnston, Stephen Albert
N1 - Funding Information:
We thank Qihua Sun and Mary Chien for excellent technical help and Irene Rombel for comments. This work was supported by grants from Bayer to BK and SAJ, a grant from DARPA to SAJ and unrestricted CBI funds to SAJ.
PY - 2005/4/27
Y1 - 2005/4/27
N2 - We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 (formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse challenge system. Nine gene fragments were isolated that conferred protection, with five protecting as effectively as the live-vaccine positive control. Bioinformatics approaches were unable to reconstruct isolation of these antigens. These results suggest that pathogen genomes can be functionally screened for vaccine candidate antigens in a mouse model to reveal new classes of vaccine candidate antigens that may have therapeutic efficacy across host species, disease manifestations, and delivery platforms.
AB - We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 (formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse challenge system. Nine gene fragments were isolated that conferred protection, with five protecting as effectively as the live-vaccine positive control. Bioinformatics approaches were unable to reconstruct isolation of these antigens. These results suggest that pathogen genomes can be functionally screened for vaccine candidate antigens in a mouse model to reveal new classes of vaccine candidate antigens that may have therapeutic efficacy across host species, disease manifestations, and delivery platforms.
KW - Chlamydia
KW - Expression library immunization
KW - Vaccine
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U2 - 10.1016/j.vaccine.2004.12.013
DO - 10.1016/j.vaccine.2004.12.013
M3 - Article
C2 - 15811648
AN - SCOPUS:16244370701
SN - 0264-410X
VL - 23
SP - 3016
EP - 3025
JO - Vaccine
JF - Vaccine
IS - 23
ER -