Screening the whole genome of a pathogen in vivo for individual protective antigens

Katherine Stemke-Hale, Bernhard Kaltenboeck, Fred J. DeGraves, Kathryn F. Sykes, Jin Huang, Chun Hui Bu, Stephen Albert Johnston

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 (formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse challenge system. Nine gene fragments were isolated that conferred protection, with five protecting as effectively as the live-vaccine positive control. Bioinformatics approaches were unable to reconstruct isolation of these antigens. These results suggest that pathogen genomes can be functionally screened for vaccine candidate antigens in a mouse model to reveal new classes of vaccine candidate antigens that may have therapeutic efficacy across host species, disease manifestations, and delivery platforms.

Original languageEnglish (US)
Pages (from-to)3016-3025
Number of pages10
JournalVaccine
Volume23
Issue number23
DOIs
StatePublished - Apr 27 2005
Externally publishedYes

Keywords

  • Chlamydia
  • Expression library immunization
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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