Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold

D. Rekesh; Y. Lyubchenko; L. S. Shlyakhtenko; Stuart Lindsay

doi:10.1016/S0006-3495(96)79308-6

Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold

D. Rekesh, Y. Lyubchenko, L. S. Shlyakhtenko, Stuart Lindsay

Research output: Contribution to journal › Article › peer-review

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Abstract

6-mercapto hexyl-oligonucleotides bind to a gold surface strongly enough to permit imaging by a scanning tunneling microscope (STM). STM images showed worm-like chains that were approximately 12-Å-wide for single-stranded DNA and 20-Å-wide for double-stranded DNA. The chain lengths corresponded to 3.4 ± 0.4 Å per basepair for double-stranded DNA and 2.2 ± 0.4 Å per base for single-stranded DNA. This unexpectedly short length for single-stranded DNA was confirmed using oligomers with both single- and double-stranded regions. When the attachment of the samples was weakened (by imaging in water or scraping with the STM tip) the images changed to pairs of 'blobs,' apparently reflecting the attachment points of the molecules to the gold surface. Given this interpretation, images of DNA containing a five-base bulge imply that the bulge bends the oligomer by 90° ± 20°.

Original language	English (US)
Pages (from-to)	1079-1086
Number of pages	8
Journal	Biophysical journal
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(96)79308-6
State	Published - Aug 1996

ASJC Scopus subject areas

Biophysics

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@article{021743985dc94e76b0bf5ba35f0daba3,

title = "Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold",

abstract = "6-mercapto hexyl-oligonucleotides bind to a gold surface strongly enough to permit imaging by a scanning tunneling microscope (STM). STM images showed worm-like chains that were approximately 12-{\AA}-wide for single-stranded DNA and 20-{\AA}-wide for double-stranded DNA. The chain lengths corresponded to 3.4 ± 0.4 {\AA} per basepair for double-stranded DNA and 2.2 ± 0.4 {\AA} per base for single-stranded DNA. This unexpectedly short length for single-stranded DNA was confirmed using oligomers with both single- and double-stranded regions. When the attachment of the samples was weakened (by imaging in water or scraping with the STM tip) the images changed to pairs of 'blobs,' apparently reflecting the attachment points of the molecules to the gold surface. Given this interpretation, images of DNA containing a five-base bulge imply that the bulge bends the oligomer by 90° ± 20°.",

author = "D. Rekesh and Y. Lyubchenko and Shlyakhtenko, {L. S.} and Stuart Lindsay",

note = "Funding Information: This work was supported by the National Institutes of Health grant 5R21HG00818-OlAI and ONR grant N00014-90-J-1455. We thank Molecular Imaging Corporation for the use of microscopes, tips, and sub- strates.",

year = "1996",

month = aug,

doi = "10.1016/S0006-3495(96)79308-6",

language = "English (US)",

volume = "71",

pages = "1079--1086",

journal = "Biophysical journal",

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T1 - Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold

AU - Rekesh, D.

AU - Lyubchenko, Y.

AU - Shlyakhtenko, L. S.

AU - Lindsay, Stuart

N1 - Funding Information: This work was supported by the National Institutes of Health grant 5R21HG00818-OlAI and ONR grant N00014-90-J-1455. We thank Molecular Imaging Corporation for the use of microscopes, tips, and sub- strates.

PY - 1996/8

Y1 - 1996/8

N2 - 6-mercapto hexyl-oligonucleotides bind to a gold surface strongly enough to permit imaging by a scanning tunneling microscope (STM). STM images showed worm-like chains that were approximately 12-Å-wide for single-stranded DNA and 20-Å-wide for double-stranded DNA. The chain lengths corresponded to 3.4 ± 0.4 Å per basepair for double-stranded DNA and 2.2 ± 0.4 Å per base for single-stranded DNA. This unexpectedly short length for single-stranded DNA was confirmed using oligomers with both single- and double-stranded regions. When the attachment of the samples was weakened (by imaging in water or scraping with the STM tip) the images changed to pairs of 'blobs,' apparently reflecting the attachment points of the molecules to the gold surface. Given this interpretation, images of DNA containing a five-base bulge imply that the bulge bends the oligomer by 90° ± 20°.

AB - 6-mercapto hexyl-oligonucleotides bind to a gold surface strongly enough to permit imaging by a scanning tunneling microscope (STM). STM images showed worm-like chains that were approximately 12-Å-wide for single-stranded DNA and 20-Å-wide for double-stranded DNA. The chain lengths corresponded to 3.4 ± 0.4 Å per basepair for double-stranded DNA and 2.2 ± 0.4 Å per base for single-stranded DNA. This unexpectedly short length for single-stranded DNA was confirmed using oligomers with both single- and double-stranded regions. When the attachment of the samples was weakened (by imaging in water or scraping with the STM tip) the images changed to pairs of 'blobs,' apparently reflecting the attachment points of the molecules to the gold surface. Given this interpretation, images of DNA containing a five-base bulge imply that the bulge bends the oligomer by 90° ± 20°.

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Scanning tunneling microscopy of mercapto-hexyl-oligonucleotides attached to gold

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