We present a method to fabricate, assemble dope-coded biosensing particles, and demonstrate a scalable high throughput protein detection application. The coded biosensing particles (8 μm in diameter and 280 nm thick) are composed of biosensing/coding/magnetic/adhesive layers and coded via patterned boron doping. Coding via doping is powerful in that it can be easily decoded, permits scalability of bit sizes down to tens of nanometers, generates a large number of codes, and retains uniform particle size and shape independent of particle code for consistent protein analysis. Following suspension phase protein binding, the dope-coded biosensing particles are extracted with an external magnet and analyzed with an atomic force microscope.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)