Sandwich immunosensing method for measuring cardiac troponin I with quantum dots-coupled antibody against troponin I

Jian Song, Jia Fan, Da Qian Song, Li Rong Bi, Gang Yu Zhou, Hao Zhang, Jing Yan Wei, Bai Yang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In this study, a novel quantitative sandwich immunoassay for detecting cTn I was developed by using surface plasmon resonance biosensor and quantum dots(QD)-coupled antibody for the first time, which amplified the response of biosensor and so had the sensitivity of this method obviously improved. First quantum dots were coupled to the monoclonal antibody(2F11) against cTn I with N-hydroxysuccinimide(NHS) and 1-ethyl-3-(3-dimethyl-aminopropyl)-carbodimidehydrochloride(EDC), and then the coupled product(QD-2F11) was confirmed by SDS-PAGE and its antibody activity was tested by protein blot. Finally, a sensitive sandwich immunosensing method for measuring cardiac troponin I was developed by surface plasmon resonance biosensor with protein A as an intermediate layer, the specific polyclonal antibody as the first antibody[capture antibody, and QD-labeled-monoclonal antibody as the second antibody(detecting antibody)]. The novel sandwich immunoassay showed the detection range of 0.4 to 15 μg/L and a detection limit of 0.4 μg/L, which is higher 2 times than the sandwich immunoassay without using QD-labeled antibody and 10 times than the direct immunoassay for cTn I previously developed, respectively.

Original languageEnglish (US)
Pages (from-to)1940-1944
Number of pages5
JournalGaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities
Volume30
Issue number10
StatePublished - Oct 2009

Keywords

  • Biosensor
  • Cardiac troponin I
  • Quantum dot
  • Sandwich immunosensing method
  • Specific antibody against cardiac troponin I

ASJC Scopus subject areas

  • Chemistry(all)

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