Sample preparation in glass capillaries for high-throughput biochemical analyses

Deirdre R. Meldrum, Mark R. Holl, Charles H. Fisher, Mohan S. Saini, Shawn K. McGuire, Timothy T.H. Ren, William H. Pence, Stephen E. Moody, David L. Cunningham, Douglas A. Donaldson, Peter J. Wiktor

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

A capillary-based fluid handling system, the ACAPELLA-5K (A5K), has been developed to prepare precise mixtures of sample and reagents in glass capillaries at a rate of 5000 preparations in 8 hours. Following a user-defined protocol the system architecture enables sample aspiration, reagent dispensing, mixing, and thermal cycling, with intermediate imaging steps for in-process monitoring of critical fluid delivery steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the reactions prepared. A typical reaction comprises a 0.5 μL sample aspirated from a microplate well followed by 1 to 8 reagents dispensed in 40-100 pL droplet volumes using piezoelectric reagent dispensers. Reactions are then mixed to prepare a 2.0 μL final reaction volume. Reaction volumes from 0.5-2 μL have been demonstrated, representing performance comparable to the state-of-the-art in a majority of core sequencing facilities. A5K has been extensively tested in the preparation of deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) and DNA sequencing reactions. Other applications of the A5K platform of technologies include quantitation of minimal residual disease, protein crystallography, and potential for application in drug discovery, forensics, and DNA analysis of environmental samples.

Original languageEnglish (US)
Title of host publicationProceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
Pages7-12
Number of pages6
DOIs
StatePublished - Dec 1 2005
Externally publishedYes
Event2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005 - Edmonton, Canada
Duration: Aug 1 2005Aug 2 2005

Publication series

NameProceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
Volume2005

Other

Other2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
CountryCanada
CityEdmonton
Period8/1/058/2/05

Keywords

  • Biomechatronics
  • Laboratory automation
  • Microfluidics
  • Sample preparation

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Meldrum, D. R., Holl, M. R., Fisher, C. H., Saini, M. S., McGuire, S. K., Ren, T. T. H., Pence, W. H., Moody, S. E., Cunningham, D. L., Donaldson, D. A., & Wiktor, P. J. (2005). Sample preparation in glass capillaries for high-throughput biochemical analyses. In Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005 (pp. 7-12). [1506737] (Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005; Vol. 2005). https://doi.org/10.1109/COASE.2005.1506737