@inbook{e3cb39c9cfa14a709f9b0972e1df8245,
title = "Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid–Protein Interaction",
abstract = "Electron crystallography is a unique tool to study membrane protein structures and lipid–protein interactions in their native-like environments. Two-dimensional (2D) protein crystallization enables the lipids immobilized by the proteins, and the generated high-resolution density map allows us to model the atomic coordinates of the surrounding lipids to study lipid–protein interaction. This protocol describes the sample preparation for electron crystallographic studies, including back-injection method and carbon sandwich method. The protocols of data collection for electron crystallography, including electron imaging and diffraction, of the 2D membrane crystal will be followed.",
keywords = "2D Crystals, Back-injection method, Carbon-sandwich method, Electron crystallography, Electron diffraction, Sugar embedding",
author = "Chan, {Ka Yi} and {Du Truong}, Chloe and Poh, {Yu Ping} and Chiu, {Po Lin}",
note = "Funding Information: We thank the Eyring Materials Center (EMC) at Arizona State University (ASU) for providing the resources. Electron crystallographic data of the AQP0 and MloK1 2D crystals was the work in Dr. Thomas Walz and Dr. Henning Stahlberg laboratories supported by the NIH grants, respectively. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0966-8_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "247--265",
booktitle = "Methods in Molecular Biology",
}