Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction

Ka Yi Chan, Chloe Du Truong, Yu Ping Poh, Po Lin Chiu

Research output: Contribution to journalArticlepeer-review

Abstract

Electron crystallography is a unique tool to study membrane protein structures and lipid-protein interactions in their native-like environments. Two-dimensional (2D) protein crystallization enables the lipids immobilized by the proteins, and the generated high-resolution density map allows us to model the atomic coordinates of the surrounding lipids to study lipid-protein interaction. This protocol describes the sample preparation for electron crystallographic studies, including back-injection method and carbon sandwich method. The protocols of data collection for electron crystallography, including electron imaging and diffraction, of the 2D membrane crystal will be followed.

Original languageEnglish (US)
Pages (from-to)247-265
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume2215
DOIs
StatePublished - 2021

Keywords

  • 2D Crystals
  • Back-injection method
  • Carbon-sandwich method
  • Electron crystallography
  • Electron diffraction
  • Sugar embedding

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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