Sample consumption reduction for serial crystallography using water-in-oil droplets

Austin Echelmeier; Jorvani Cruz Villarreal; Daihyn Kim; Sahir Gandhi; Ana Egatz-Gomez; Darren Thifault; Jesse D. Coe; Gerrit Brehm; Caleb Madsen; Sebastian Quintana; Saša Bajt; Marc Messerschmidt; Jose Domingo Meza-Aguilar; Dominik Oberthür; Max O. Wiedorn; Holger Fleckenstein; Sabine Botha; Derek Mendez; Juraj Knoska; Jose Martin Garcia; Hao Hu; Stella Lisova; Aschkan Allah Gholi; Yaroslav Gevorkov; Kartik Ayyer; Steve J. Aplin; Helen M. Ginn; Heinz Graafsma; Andrew J. Morgan; Dominc Greiffenberg; Alexander Klujev; Torsten Laurus; Jennifer Poehlsen; Ulrich Trunk; Filipe R.N.C. Maia; Davide Mezza; Raimund Fromme; Britta Weinhausen; Grant Mills; Patrik Vagovic; Yoonhee Kim; Joachim Schulz; Katerina Dörner; Jolanta Sztuk-Dambietz; Manuela Kuhn; Thomas D. Grant; Thomas A. White; Valerio Mariani; Anton Barty; Adrian P. Mancuso; Uwe Weierstall; John C.H. Spence; Henry N. Chapman; Nadia A. Zatsepin; Petra Fromme; Richard A. Kirian; Alexandra Ros

Sample consumption reduction for serial crystallography using water-in-oil droplets

Austin Echelmeier, Jorvani Cruz Villarreal, Daihyn Kim, Sahir Gandhi, Ana Egatz-Gomez, Darren Thifault, Jesse D. Coe, Gerrit Brehm, Caleb Madsen, Sebastian Quintana, Saša Bajt, Marc Messerschmidt, Jose Domingo Meza-Aguilar, Dominik Oberthür, Max O. Wiedorn, Holger Fleckenstein, Sabine Botha, Derek Mendez, Juraj Knoska, Jose Martin GarciaHao Hu, Stella Lisova, Aschkan Allah Gholi, Yaroslav Gevorkov, Kartik Ayyer, Steve J. Aplin, Helen M. Ginn, Heinz Graafsma, Andrew J. Morgan, Dominc Greiffenberg, Alexander Klujev, Torsten Laurus, Jennifer Poehlsen, Ulrich Trunk, Filipe R.N.C. Maia, Davide Mezza, Raimund Fromme, Britta Weinhausen, Grant Mills, Patrik Vagovic, Yoonhee Kim, Joachim Schulz, Katerina Dörner, Jolanta Sztuk-Dambietz, Manuela Kuhn, Thomas D. Grant, Thomas A. White, Valerio Mariani, Anton Barty, Adrian P. Mancuso, Uwe Weierstall, John C.H. Spence, Henry N. Chapman, Nadia A. Zatsepin, Petra Fromme, Richard A. Kirian, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Serial femtosecond crystallography with an X-ray free electron laser (XFEL) wastes up to 99% of the continuously injected sample due to the XFEL pulse structure. To reduce sample waste, we utilize a 3D printed microfluidic droplet generator coupled to an injection nozzle to deliver a segmented flow containing droplets of aqueous crystal suspension within an oil carrier. The structure of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) was determined and sample waste reduced significantly.

Original language	English (US)
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	Chemical and Biological Microsystems Society
Pages	1476-1477
Number of pages	2
ISBN (Electronic)	9781733419000
State	Published - 2019
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: Oct 27 2019 → Oct 31 2019

Publication series

Name	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	10/27/19 → 10/31/19

Keywords

3D Printing
Crystallography
Droplets
Microfluidics
SFX
XFEL

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

Echelmeier, A., Villarreal, J. C., Kim, D., Gandhi, S., Egatz-Gomez, A., Thifault, D., Coe, J. D., Brehm, G., Madsen, C., Quintana, S., Bajt, S., Messerschmidt, M., Meza-Aguilar, J. D., Oberthür, D., Wiedorn, M. O., Fleckenstein, H., Botha, S., Mendez, D., Knoska, J., ... Ros, A. (2019). Sample consumption reduction for serial crystallography using water-in-oil droplets. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 1476-1477). (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019). Chemical and Biological Microsystems Society.

Sample consumption reduction for serial crystallography using water-in-oil droplets. / Echelmeier, Austin; Villarreal, Jorvani Cruz; Kim, Daihyn et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. p. 1476-1477 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Echelmeier, A, Villarreal, JC, Kim, D, Gandhi, S, Egatz-Gomez, A, Thifault, D, Coe, JD, Brehm, G, Madsen, C, Quintana, S, Bajt, S, Messerschmidt, M, Meza-Aguilar, JD, Oberthür, D, Wiedorn, MO, Fleckenstein, H, Botha, S, Mendez, D, Knoska, J, Garcia, JM, Hu, H, Lisova, S, Gholi, AA, Gevorkov, Y, Ayyer, K, Aplin, SJ, Ginn, HM, Graafsma, H, Morgan, AJ, Greiffenberg, D, Klujev, A, Laurus, T, Poehlsen, J, Trunk, U, Maia, FRNC, Mezza, D, Fromme, R, Weinhausen, B, Mills, G, Vagovic, P, Kim, Y, Schulz, J, Dörner, K, Sztuk-Dambietz, J, Kuhn, M, Grant, TD, White, TA, Mariani, V, Barty, A, Mancuso, AP, Weierstall, U, Spence, JCH, Chapman, HN, Zatsepin, NA, Fromme, P , Kirian, RA & Ros, A 2019, Sample consumption reduction for serial crystallography using water-in-oil droplets. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Chemical and Biological Microsystems Society, pp. 1476-1477, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, Switzerland, 10/27/19.

Echelmeier A, Villarreal JC, Kim D, Gandhi S, Egatz-Gomez A, Thifault D et al. Sample consumption reduction for serial crystallography using water-in-oil droplets. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society. 2019. p. 1476-1477. (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

Echelmeier, Austin ; Villarreal, Jorvani Cruz ; Kim, Daihyn et al. / Sample consumption reduction for serial crystallography using water-in-oil droplets. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. pp. 1476-1477 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

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title = "Sample consumption reduction for serial crystallography using water-in-oil droplets",

abstract = "Serial femtosecond crystallography with an X-ray free electron laser (XFEL) wastes up to 99% of the continuously injected sample due to the XFEL pulse structure. To reduce sample waste, we utilize a 3D printed microfluidic droplet generator coupled to an injection nozzle to deliver a segmented flow containing droplets of aqueous crystal suspension within an oil carrier. The structure of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) was determined and sample waste reduced significantly.",

keywords = "3D Printing, Crystallography, Droplets, Microfluidics, SFX, XFEL",

author = "Austin Echelmeier and Villarreal, {Jorvani Cruz} and Daihyn Kim and Sahir Gandhi and Ana Egatz-Gomez and Darren Thifault and Coe, {Jesse D.} and Gerrit Brehm and Caleb Madsen and Sebastian Quintana and Sa{\v s}a Bajt and Marc Messerschmidt and Meza-Aguilar, {Jose Domingo} and Dominik Oberth{\"u}r and Wiedorn, {Max O.} and Holger Fleckenstein and Sabine Botha and Derek Mendez and Juraj Knoska and Garcia, {Jose Martin} and Hao Hu and Stella Lisova and Gholi, {Aschkan Allah} and Yaroslav Gevorkov and Kartik Ayyer and Aplin, {Steve J.} and Ginn, {Helen M.} and Heinz Graafsma and Morgan, {Andrew J.} and Dominc Greiffenberg and Alexander Klujev and Torsten Laurus and Jennifer Poehlsen and Ulrich Trunk and Maia, {Filipe R.N.C.} and Davide Mezza and Raimund Fromme and Britta Weinhausen and Grant Mills and Patrik Vagovic and Yoonhee Kim and Joachim Schulz and Katerina D{\"o}rner and Jolanta Sztuk-Dambietz and Manuela Kuhn and Grant, {Thomas D.} and White, {Thomas A.} and Valerio Mariani and Anton Barty and Mancuso, {Adrian P.} and Uwe Weierstall and Spence, {John C.H.} and Chapman, {Henry N.} and Zatsepin, {Nadia A.} and Petra Fromme and Kirian, {Richard A.} and Alexandra Ros",

note = "Funding Information: The work was financially supported by the STC Program of the National Science Foundation through BioXFEL under Agreement No. 1231306 and the National Institutes of Health Award No. R01GM095583. Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "English (US)",

series = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

pages = "1476--1477",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

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TY - GEN

T1 - Sample consumption reduction for serial crystallography using water-in-oil droplets

AU - Echelmeier, Austin

AU - Villarreal, Jorvani Cruz

AU - Kim, Daihyn

AU - Gandhi, Sahir

AU - Egatz-Gomez, Ana

AU - Thifault, Darren

AU - Coe, Jesse D.

AU - Brehm, Gerrit

AU - Madsen, Caleb

AU - Quintana, Sebastian

AU - Bajt, Saša

AU - Messerschmidt, Marc

AU - Meza-Aguilar, Jose Domingo

AU - Oberthür, Dominik

AU - Wiedorn, Max O.

AU - Fleckenstein, Holger

AU - Botha, Sabine

AU - Mendez, Derek

AU - Knoska, Juraj

AU - Garcia, Jose Martin

AU - Hu, Hao

AU - Lisova, Stella

AU - Gholi, Aschkan Allah

AU - Gevorkov, Yaroslav

AU - Ayyer, Kartik

AU - Aplin, Steve J.

AU - Ginn, Helen M.

AU - Graafsma, Heinz

AU - Morgan, Andrew J.

AU - Greiffenberg, Dominc

AU - Klujev, Alexander

AU - Laurus, Torsten

AU - Poehlsen, Jennifer

AU - Trunk, Ulrich

AU - Maia, Filipe R.N.C.

AU - Mezza, Davide

AU - Fromme, Raimund

AU - Weinhausen, Britta

AU - Mills, Grant

AU - Vagovic, Patrik

AU - Kim, Yoonhee

AU - Schulz, Joachim

AU - Dörner, Katerina

AU - Sztuk-Dambietz, Jolanta

AU - Kuhn, Manuela

AU - Grant, Thomas D.

AU - White, Thomas A.

AU - Mariani, Valerio

AU - Barty, Anton

AU - Mancuso, Adrian P.

AU - Weierstall, Uwe

AU - Spence, John C.H.

AU - Chapman, Henry N.

AU - Zatsepin, Nadia A.

AU - Fromme, Petra

AU - Kirian, Richard A.

AU - Ros, Alexandra

N1 - Funding Information: The work was financially supported by the STC Program of the National Science Foundation through BioXFEL under Agreement No. 1231306 and the National Institutes of Health Award No. R01GM095583. Publisher Copyright: © 2019 CBMS-0001.

PY - 2019

Y1 - 2019

N2 - Serial femtosecond crystallography with an X-ray free electron laser (XFEL) wastes up to 99% of the continuously injected sample due to the XFEL pulse structure. To reduce sample waste, we utilize a 3D printed microfluidic droplet generator coupled to an injection nozzle to deliver a segmented flow containing droplets of aqueous crystal suspension within an oil carrier. The structure of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) was determined and sample waste reduced significantly.

AB - Serial femtosecond crystallography with an X-ray free electron laser (XFEL) wastes up to 99% of the continuously injected sample due to the XFEL pulse structure. To reduce sample waste, we utilize a 3D printed microfluidic droplet generator coupled to an injection nozzle to deliver a segmented flow containing droplets of aqueous crystal suspension within an oil carrier. The structure of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) was determined and sample waste reduced significantly.

KW - 3D Printing

KW - Crystallography

KW - Droplets

KW - Microfluidics

KW - SFX

KW - XFEL

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UR - http://www.scopus.com/inward/citedby.url?scp=85094945437&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85094945437

T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

SP - 1476

EP - 1477

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Sample consumption reduction for serial crystallography using water-in-oil droplets

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

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Cite this