Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene

Heinz U. Gläser, Dominique Thomas, Roberto Gaxiola, Françoise Montrichard, Yolande Surdin-Kerjan, Ramón Serrano

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The progressive salinization of irrigated land poses a threat to the future of agriculture in arid regions. The identification of crucial metabolic steps in salt tolerance is important for the understanding of stress physiology and may provide the tools for its genetic engineering. In the yeast Saccharomyces cerevisiae we have isolated a gene, HAL2, which upon increase in gene dosage improves growth under NaCl and LiCl stresses. The HAL2 protein is homologous to inositol phosphatases, enzymes known to be inhibited by lithium salts. Complementation analysis demonstrated that HAL2 is identical to MET22, a gene involved in methionine biosynthesis. Accordingly, methionine supplementation improves the tolerance of yeast to NaCl and LiCl. These results demonstrate an unsuspected interplay between methionine biosynthesis and salt tolerance.

Original languageEnglish (US)
Pages (from-to)3105-3110
Number of pages6
JournalEMBO Journal
Volume12
Issue number8
StatePublished - 1993
Externally publishedYes

Fingerprint

Salt-Tolerance
Biosynthesis
Phosphoric Monoester Hydrolases
Methionine
Yeast
Saccharomyces cerevisiae
Salts
Genes
Yeasts
Genetic engineering
Arid regions
Genetic Engineering
Gene Dosage
Physiology
Agriculture
Lithium
Enzymes
Growth
Proteins

Keywords

  • Methionine
  • Phosphatase
  • Salt tolerance
  • Yeast

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Gläser, H. U., Thomas, D., Gaxiola, R., Montrichard, F., Surdin-Kerjan, Y., & Serrano, R. (1993). Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. EMBO Journal, 12(8), 3105-3110.

Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. / Gläser, Heinz U.; Thomas, Dominique; Gaxiola, Roberto; Montrichard, Françoise; Surdin-Kerjan, Yolande; Serrano, Ramón.

In: EMBO Journal, Vol. 12, No. 8, 1993, p. 3105-3110.

Research output: Contribution to journalArticle

Gläser, HU, Thomas, D, Gaxiola, R, Montrichard, F, Surdin-Kerjan, Y & Serrano, R 1993, 'Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene', EMBO Journal, vol. 12, no. 8, pp. 3105-3110.
Gläser HU, Thomas D, Gaxiola R, Montrichard F, Surdin-Kerjan Y, Serrano R. Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. EMBO Journal. 1993;12(8):3105-3110.
Gläser, Heinz U. ; Thomas, Dominique ; Gaxiola, Roberto ; Montrichard, Françoise ; Surdin-Kerjan, Yolande ; Serrano, Ramón. / Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. In: EMBO Journal. 1993 ; Vol. 12, No. 8. pp. 3105-3110.
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