TY - JOUR
T1 - Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls
AU - Lohavanichbutr, Pawadee
AU - Zhang, Yuzheng
AU - Wang, Pei
AU - Gu, Haiwei
AU - Nagana Gowda, G. A.
AU - Djukovic, Danijel
AU - Buas, Matthew F.
AU - Raftery, Daniel
AU - Chen, Chu
N1 - Funding Information:
This study was supported by grants R01 CA095419, R21 CA187151, and P30CA015704-40 from the National Cancer Institute, National Institutes of Health and funds from the Fred Hutchinson Cancer Research Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank the study participants and their families for their contribution to this study.
Publisher Copyright:
© 2018 Lohavanichbutr et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/9
Y1 - 2018/9
N2 - Oral cavity squamous cell carcinoma (OCC) and oropharyngeal squamous cell carcinoma (OPC) are among the most common cancers worldwide and are associated with high mortality and morbidity. The purpose of this study is to identify potential biomarkers to distinguish OCC/OPC from normal controls and to distinguish OCC patients with and without nodal metastasis. We tested saliva samples from 101 OCC, 58 OPC, and 35 normal controls using four analytical platforms (NMR, targeted aqueous by LC-MS/MS, global aqueous and global lipidomics by LC-Q-TOF). Samples from OCC and normal controls were divided into discovery and validation sets. Using linear regression adjusting for age, sex, race and experimental batches, we found the levels of two metabolites (glycine and proline) to be significantly different between OCC and controls (FDR < 0.1 for both discovery and validation sets) but did not find any appreciable differences in metabolite levels between OPC and controls or between OCC with and without nodal metastasis. Four metabolites, including glycine, proline, citrulline, and ornithine were associated with early stage OCC in both discovery and validation sets. Further study is warranted to confirm these results in the development of salivary metabolites as diagnostic markers.
AB - Oral cavity squamous cell carcinoma (OCC) and oropharyngeal squamous cell carcinoma (OPC) are among the most common cancers worldwide and are associated with high mortality and morbidity. The purpose of this study is to identify potential biomarkers to distinguish OCC/OPC from normal controls and to distinguish OCC patients with and without nodal metastasis. We tested saliva samples from 101 OCC, 58 OPC, and 35 normal controls using four analytical platforms (NMR, targeted aqueous by LC-MS/MS, global aqueous and global lipidomics by LC-Q-TOF). Samples from OCC and normal controls were divided into discovery and validation sets. Using linear regression adjusting for age, sex, race and experimental batches, we found the levels of two metabolites (glycine and proline) to be significantly different between OCC and controls (FDR < 0.1 for both discovery and validation sets) but did not find any appreciable differences in metabolite levels between OPC and controls or between OCC with and without nodal metastasis. Four metabolites, including glycine, proline, citrulline, and ornithine were associated with early stage OCC in both discovery and validation sets. Further study is warranted to confirm these results in the development of salivary metabolites as diagnostic markers.
UR - http://www.scopus.com/inward/record.url?scp=85053687500&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053687500&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0204249
DO - 10.1371/journal.pone.0204249
M3 - Article
C2 - 30235319
AN - SCOPUS:85053687500
VL - 13
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 9
M1 - e0204249
ER -