Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens

Arindam Mitra, Amanda Loh, Amanda Gonzales, Paweł Łaniewski, Crystal Willingham, Roy Curtiss, Kenneth L. Roland

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Salmonella enterica serovar Gallinarum is the causative agent of fowl typhoid, an important systemic disease of poultry with economic consequences in developing nations. A live attenuated orally applied S. Gallinarum vaccine could provide a low cost method for controlling this disease. We constructed S. Gallinarum strains in which the expression of the crp, rfc and rfaH genes, important for virulence of Salmonella Typhimurium in mice, were under the control of an arabinose-regulated promoter. We evaluated the virulence of these strains compared to wild-type S. Gallinarum and to mutants carrying deletions in these genes. We found that rfc mutants were fully virulent, indicating that, unlike the S. Typhimurium mouse model, the rfc gene is dispensable in S. Gallinarum for virulence in birds. In the case of rfaH, the deletion mutant was attenuated and protective, while the strain with arabinose-regulated rfaH expression retained full virulence. The strain exhibiting arabinose-regulated crp expression was attenuated. Its virulence was not affected by the inclusion of 0.2% arabinose in the drinking water. Birds immunized with this strain were protected against a lethal S. Gallinarum challenge and against colonization with the human pathogen Salmonella Enteritidis. This work shows that an arabinose-regulated crp strain provides a basis for further development of a fowl typhoid vaccine.

Original languageEnglish (US)
Pages (from-to)1094-1099
Number of pages6
JournalVaccine
Volume31
Issue number7
DOIs
StatePublished - Feb 4 2013

Fingerprint

Rhode Island Red
Salmonella Gallinarum
Arabinose
Salmonella
Virulence
arabinose
Chickens
chickens
virulence
Safety
mutants
fowl typhoid
Salmonella typhimurium
Birds
Salmonella Typhimurium
Poultry Diseases
Typhoid-Paratyphoid Vaccines
Salmonella enteritidis
Salmonella enterica
vaccines

Keywords

  • Fowl typhoid
  • Lipopolysaccharides
  • Live attenuated vaccines
  • Poultry
  • Regulated delayed attenuation

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Mitra, A., Loh, A., Gonzales, A., Łaniewski, P., Willingham, C., Curtiss, R., & Roland, K. L. (2013). Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens. Vaccine, 31(7), 1094-1099. https://doi.org/10.1016/j.vaccine.2012.12.021

Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens. / Mitra, Arindam; Loh, Amanda; Gonzales, Amanda; Łaniewski, Paweł; Willingham, Crystal; Curtiss, Roy; Roland, Kenneth L.

In: Vaccine, Vol. 31, No. 7, 04.02.2013, p. 1094-1099.

Research output: Contribution to journalArticle

Mitra, A, Loh, A, Gonzales, A, Łaniewski, P, Willingham, C, Curtiss, R & Roland, KL 2013, 'Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens', Vaccine, vol. 31, no. 7, pp. 1094-1099. https://doi.org/10.1016/j.vaccine.2012.12.021
Mitra, Arindam ; Loh, Amanda ; Gonzales, Amanda ; Łaniewski, Paweł ; Willingham, Crystal ; Curtiss, Roy ; Roland, Kenneth L. / Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens. In: Vaccine. 2013 ; Vol. 31, No. 7. pp. 1094-1099.
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