Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients

Russell Dorer, Peter M. Pryciak, Leland Hartwell

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

During conjugation, haploid S. cerevisiae cells find one another by polarizing their growth toward each other along gradients of pheromone (chemotropism). We demonstrate that yeast cells exhibit a second mating behavior: when their receptors are saturated with pheromone, wild-type a cells execute a default pathway and select a mate at random. These matings are less efficient than chemotropic matings, are induced by the same dose of pheromone that induces shmoo formation, and appear to use a site near the incipient bud site for polarization. We show that the SPA2 gent is specifically required for the default pathway: spa2Δ mutants cannot mate if pheromone concentrations are high and gradients are absent, but can mate if gradients are present. ste2Δ, sst2Δ, and far1Δ mutants are chemotropism- defective and therefore must choose a mate by using a default pathway; consistent with this deduction, these strains require SPA2 to mate. In addition, our results suggest that far1 mutants are chemotropism-defective because their mating polarity is fixed at the incipient bud site, suggesting that the FAR1 gene is required for inhibiting the use of the incipient bud site during chemotropic mating. These observations reveal a molecular relationship between the mating and budding polarity pathways.

Original languageEnglish (US)
Pages (from-to)845-861
Number of pages17
JournalJournal of Cell Biology
Volume131
Issue number4
DOIs
StatePublished - Nov 1995
Externally publishedYes

Fingerprint

Pheromones
Saccharomyces cerevisiae
Haploidy
Yeasts
Growth
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients. / Dorer, Russell; Pryciak, Peter M.; Hartwell, Leland.

In: Journal of Cell Biology, Vol. 131, No. 4, 11.1995, p. 845-861.

Research output: Contribution to journalArticle

@article{a40270a097a24a168bd12320a167663c,
title = "Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients",
abstract = "During conjugation, haploid S. cerevisiae cells find one another by polarizing their growth toward each other along gradients of pheromone (chemotropism). We demonstrate that yeast cells exhibit a second mating behavior: when their receptors are saturated with pheromone, wild-type a cells execute a default pathway and select a mate at random. These matings are less efficient than chemotropic matings, are induced by the same dose of pheromone that induces shmoo formation, and appear to use a site near the incipient bud site for polarization. We show that the SPA2 gent is specifically required for the default pathway: spa2Δ mutants cannot mate if pheromone concentrations are high and gradients are absent, but can mate if gradients are present. ste2Δ, sst2Δ, and far1Δ mutants are chemotropism- defective and therefore must choose a mate by using a default pathway; consistent with this deduction, these strains require SPA2 to mate. In addition, our results suggest that far1 mutants are chemotropism-defective because their mating polarity is fixed at the incipient bud site, suggesting that the FAR1 gene is required for inhibiting the use of the incipient bud site during chemotropic mating. These observations reveal a molecular relationship between the mating and budding polarity pathways.",
author = "Russell Dorer and Pryciak, {Peter M.} and Leland Hartwell",
year = "1995",
month = "11",
doi = "10.1083/jcb.131.4.845",
language = "English (US)",
volume = "131",
pages = "845--861",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

TY - JOUR

T1 - Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients

AU - Dorer, Russell

AU - Pryciak, Peter M.

AU - Hartwell, Leland

PY - 1995/11

Y1 - 1995/11

N2 - During conjugation, haploid S. cerevisiae cells find one another by polarizing their growth toward each other along gradients of pheromone (chemotropism). We demonstrate that yeast cells exhibit a second mating behavior: when their receptors are saturated with pheromone, wild-type a cells execute a default pathway and select a mate at random. These matings are less efficient than chemotropic matings, are induced by the same dose of pheromone that induces shmoo formation, and appear to use a site near the incipient bud site for polarization. We show that the SPA2 gent is specifically required for the default pathway: spa2Δ mutants cannot mate if pheromone concentrations are high and gradients are absent, but can mate if gradients are present. ste2Δ, sst2Δ, and far1Δ mutants are chemotropism- defective and therefore must choose a mate by using a default pathway; consistent with this deduction, these strains require SPA2 to mate. In addition, our results suggest that far1 mutants are chemotropism-defective because their mating polarity is fixed at the incipient bud site, suggesting that the FAR1 gene is required for inhibiting the use of the incipient bud site during chemotropic mating. These observations reveal a molecular relationship between the mating and budding polarity pathways.

AB - During conjugation, haploid S. cerevisiae cells find one another by polarizing their growth toward each other along gradients of pheromone (chemotropism). We demonstrate that yeast cells exhibit a second mating behavior: when their receptors are saturated with pheromone, wild-type a cells execute a default pathway and select a mate at random. These matings are less efficient than chemotropic matings, are induced by the same dose of pheromone that induces shmoo formation, and appear to use a site near the incipient bud site for polarization. We show that the SPA2 gent is specifically required for the default pathway: spa2Δ mutants cannot mate if pheromone concentrations are high and gradients are absent, but can mate if gradients are present. ste2Δ, sst2Δ, and far1Δ mutants are chemotropism- defective and therefore must choose a mate by using a default pathway; consistent with this deduction, these strains require SPA2 to mate. In addition, our results suggest that far1 mutants are chemotropism-defective because their mating polarity is fixed at the incipient bud site, suggesting that the FAR1 gene is required for inhibiting the use of the incipient bud site during chemotropic mating. These observations reveal a molecular relationship between the mating and budding polarity pathways.

UR - http://www.scopus.com/inward/record.url?scp=0028805208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028805208&partnerID=8YFLogxK

U2 - 10.1083/jcb.131.4.845

DO - 10.1083/jcb.131.4.845

M3 - Article

C2 - 7490289

AN - SCOPUS:0028805208

VL - 131

SP - 845

EP - 861

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -