Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae

Viktor Stolc, Sidney Altman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association with both RNase P RNA and a related endoribonuclease, RNase MRP RNA, in immunoprecipitates from crude extracts of cells. Depletion of Rpp1 in vivo leads to the accumulation of precursor tRNAs with unprocessed 5' and 3' termini and reveals rRNA processing defects that have not been described previously for proteins associated with RNase P or RNase MRP. Immunoprecipitated complexes cleave both yeast precursor tRNAs and precursor rRNAs.

Original languageEnglish (US)
Pages (from-to)2414-2425
Number of pages12
JournalGenes and Development
Volume11
Issue number18
DOIs
StatePublished - Sep 15 1997
Externally publishedYes

Keywords

  • Essential protein subunit
  • Nuclear RNase P
  • Precursor rRNA
  • Precursor tRNA
  • Rpp1
  • S. Cerevisiae

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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