Rotation of single live mammalian cells using dynamic holographic optical tweezers

Cao Bin Cao; Laimonas Kelbauskas; Samantha Chan; Rishabh M. Shetty; Dean Smith; Deirdre Meldrum

doi:10.1016/j.optlaseng.2016.12.019

Rotation of single live mammalian cells using dynamic holographic optical tweezers

Cao Bin Cao, Laimonas Kelbauskas, Samantha Chan, Rishabh M. Shetty, Dean Smith, Deirdre Meldrum

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

Original language	English (US)
Pages (from-to)	70-75
Number of pages	6
Journal	Optics and Lasers in Engineering
Volume	92
DOIs	https://doi.org/10.1016/j.optlaseng.2016.12.019
State	Published - May 1 2017

Keywords

Laser trapping
Optical manipulation
Optical tweezers
Single cells rotation
Spatial light modulation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1016/j.optlaseng.2016.12.019

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title = "Rotation of single live mammalian cells using dynamic holographic optical tweezers",

abstract = "We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.",

keywords = "Laser trapping, Optical manipulation, Optical tweezers, Single cells rotation, Spatial light modulation",

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T1 - Rotation of single live mammalian cells using dynamic holographic optical tweezers

AU - Bin Cao, Cao

AU - Kelbauskas, Laimonas

AU - Chan, Samantha

AU - Shetty, Rishabh M.

AU - Smith, Dean

AU - Meldrum, Deirdre

PY - 2017/5/1

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N2 - We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

AB - We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

KW - Laser trapping

KW - Optical manipulation

KW - Optical tweezers

KW - Single cells rotation

KW - Spatial light modulation

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Rotation of single live mammalian cells using dynamic holographic optical tweezers

Abstract

Keywords

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