TY - GEN
T1 - Rotation of cells and cell clusters in culture media for optical computed tomography
AU - Wang, Hong
AU - Stanley, Matthew
AU - Elango, Iniyan Soundappa
AU - Shetty, Rishabh M.
AU - Teller, Wacey
AU - Shabilla, Andrew
AU - Limsirichai, Pimwadee
AU - Zhu, Haixin
AU - Houkal, Jeff
AU - Smith, Dean
AU - Chao, Shih-Hui
AU - Kelbauskas, Laimonas
AU - Johnson, Roger H.
AU - Meldrum, Deirdre
PY - 2013
Y1 - 2013
N2 - We present an octupole electrocage microchip designed to rotate biological cells. The device consists of two sets of four planar electrodes fabricated on glass wafers and separated by a 60-μm-thick negative photoresist layer incorporating a microfluidic channel for cell delivery and retrieval. By application of high frequency AC voltages with appropriate phase lags, the electrocage can rotate live or fixed single cells or cell clusters about a stationary axis in the focal plane, i.e. perpendicular to the optical axis. When coupled with an imaging platform, isotropic 3D images with submicron resolution can be reconstructed from hundreds of projections acquired from various angles as cells are rotated.
AB - We present an octupole electrocage microchip designed to rotate biological cells. The device consists of two sets of four planar electrodes fabricated on glass wafers and separated by a 60-μm-thick negative photoresist layer incorporating a microfluidic channel for cell delivery and retrieval. By application of high frequency AC voltages with appropriate phase lags, the electrocage can rotate live or fixed single cells or cell clusters about a stationary axis in the focal plane, i.e. perpendicular to the optical axis. When coupled with an imaging platform, isotropic 3D images with submicron resolution can be reconstructed from hundreds of projections acquired from various angles as cells are rotated.
KW - Cell cluster
KW - Cell computed tomography (cell CT)
KW - Dielectrophoresis
KW - Single cell
UR - http://www.scopus.com/inward/record.url?scp=84907378132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907378132&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907378132
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1090
EP - 1092
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -