Rotation of cells and cell clusters in culture media for optical computed tomography

Hong Wang, Matthew Stanley, Iniyan Soundappa Elango, Rishabh M. Shetty, Wacey Teller, Andrew Shabilla, Pimwadee Limsirichai, Haixin Zhu, Jeff Houkal, Dean Smith, Shih-Hui Chao, Laimonas Kelbauskas, Roger H. Johnson, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present an octupole electrocage microchip designed to rotate biological cells. The device consists of two sets of four planar electrodes fabricated on glass wafers and separated by a 60-μm-thick negative photoresist layer incorporating a microfluidic channel for cell delivery and retrieval. By application of high frequency AC voltages with appropriate phase lags, the electrocage can rotate live or fixed single cells or cell clusters about a stationary axis in the focal plane, i.e. perpendicular to the optical axis. When coupled with an imaging platform, isotropic 3D images with submicron resolution can be reconstructed from hundreds of projections acquired from various angles as cells are rotated.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1090-1092
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

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Keywords

  • Cell cluster
  • Cell computed tomography (cell CT)
  • Dielectrophoresis
  • Single cell

ASJC Scopus subject areas

  • Bioengineering

Cite this

Wang, H., Stanley, M., Elango, I. S., Shetty, R. M., Teller, W., Shabilla, A., Limsirichai, P., Zhu, H., Houkal, J., Smith, D., Chao, S-H., Kelbauskas, L., Johnson, R. H., & Meldrum, D. (2013). Rotation of cells and cell clusters in culture media for optical computed tomography. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1090-1092). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.