Abstract
Rotary shadowing when combined with such specimen preparation techniques as quick freezing and deep etching, critical point drying, and glycerol spraying is a highly versatile method of visualizing cell and macromolecular ultrastructure. This review outlines the procedures commonly used to prepare specimens for rotary shadowing and evaluates the relative merits of rotary shadowing when compared to unidirectional shadowing and negative staining.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 305-335 |
| Number of pages | 31 |
| Journal | Journal of electron microscopy technique |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1986 |
Keywords
- Electron microscopy
- Freeze fracture
- Mast cells
- Neutrophils
- Quick freezing
- Shadowing
ASJC Scopus subject areas
- Anatomy