Rotary shadowing with platinum‐carbon in biological electron microscopy

A review of methods and applications

Douglas E. Chandler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rotary shadowing when combined with such specimen preparation techniques as quick freezing and deep etching, critical point drying, and glycerol spraying is a highly versatile method of visualizing cell and macromolecular ultrastructure. This review outlines the procedures commonly used to prepare specimens for rotary shadowing and evaluates the relative merits of rotary shadowing when compared to unidirectional shadowing and negative staining.

Original languageEnglish (US)
Pages (from-to)305-335
Number of pages31
JournalJournal of Electron Microscopy Technique
Volume3
Issue number3
DOIs
StatePublished - 1986

Fingerprint

Specimen preparation
Spraying
Glycerol
Freezing
Electron microscopy
Etching
electron microscopy
Electron Microscopy
Drying
spraying
staining
glycerols
freezing
drying
critical point
etching
Negative Staining
preparation
cells

Keywords

  • Electron microscopy
  • Freeze fracture
  • Mast cells
  • Neutrophils
  • Quick freezing
  • Shadowing

ASJC Scopus subject areas

  • Anatomy

Cite this

Rotary shadowing with platinum‐carbon in biological electron microscopy : A review of methods and applications. / Chandler, Douglas E.

In: Journal of Electron Microscopy Technique, Vol. 3, No. 3, 1986, p. 305-335.

Research output: Contribution to journalArticle

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