Rotary shadowing with platinum‐carbon in biological electron microscopy: A review of methods and applications

Douglas E. Chandler

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Rotary shadowing when combined with such specimen preparation techniques as quick freezing and deep etching, critical point drying, and glycerol spraying is a highly versatile method of visualizing cell and macromolecular ultrastructure. This review outlines the procedures commonly used to prepare specimens for rotary shadowing and evaluates the relative merits of rotary shadowing when compared to unidirectional shadowing and negative staining.

Original languageEnglish (US)
Pages (from-to)305-335
Number of pages31
JournalJournal of Electron Microscopy Technique
Volume3
Issue number3
DOIs
StatePublished - 1986

Keywords

  • Electron microscopy
  • Freeze fracture
  • Mast cells
  • Neutrophils
  • Quick freezing
  • Shadowing

ASJC Scopus subject areas

  • Anatomy

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