Role of the coronavirus E viroporin protein transmembrane domain in virus assembly

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Coronavirus envelope (E) proteins are small (∼75- to 110-amino-acid) membrane proteins that have a short hydrophilic amino terminus, a relatively long hydrophobic membrane domain, and a long hydrophilic carboxy-terminal domain. The protein is a minor virion structural component that plays an important, not fully understood role in virus production. It was recently demonstrated that the protein forms ion channels. We investigated the importance of the hydrophobic domain of the mouse hepatitis coronavirus (MHV) A59 E protein. Alanine scanning insertion mutagenesis was used to examine the effect of disruption of the domain on virus production in the context of the virus genome by using a MHV A59 infectious clone. Mutant viruses exhibited smaller plaque phenotypes, and virus production was significantly crippled. Analysis of recovered viruses suggested that the structure of the presumed α-helical structure and positioning of polar hydrophilic residues within the predicted transmembrane domain are important for virus production. Generation of viruses with restored wild-type helical pitch resulted in increased virus production, but some exhibited decreased virus release. Viruses with the restored helical pitch were more sensitive to treatment with the ion channel inhibitor hexamethylene amiloride than were the more crippled parental viruses with the single alanine insertions, suggesting that disruption of the transmembrane domain affects the functional activity of the protein. Overall the results indicate that the transmembrane domain plays a crucial role during biogenesis of virions.

Original languageEnglish (US)
Pages (from-to)3597-3607
Number of pages11
JournalJournal of Virology
Volume81
Issue number7
DOIs
StatePublished - Apr 2007

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Virus Assembly
Coronavirus
transmembrane proteins
Coronavirinae
Viruses
viruses
Proteins
Ion Channels
ion channels
hepatitis
Alanine
Virion
Hepatitis
virion
proteins
alanine
Protein Domains
virus assembly
Virus Release
viral morphology

ASJC Scopus subject areas

  • Immunology

Cite this

Role of the coronavirus E viroporin protein transmembrane domain in virus assembly. / Ye, Ye; Hogue, Brenda.

In: Journal of Virology, Vol. 81, No. 7, 04.2007, p. 3597-3607.

Research output: Contribution to journalArticle

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