Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli

Nadim Majdalani, Michael Heck, Valerie Stout, Susan Gottesman

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The rcs phosphorelay pathway components were originally identified as regulators of capsule synthesis. In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed rcsD, acts as a phosphorelay between RcsC and RcsB. Transcription of genes for capsule synthesis (cps) requires both RcsA and RcsB; transcription of other promoters, including that for the small RNA RprA, requires only RcsB. RcsF was described as an alternative sensor kinase for RcsB. We have examined the role of RcsF in the activation of both the rprA and cps promoters. We find that a number of signals that lead to activation of the phosphorelay require both RcsF and RcsC; epistasis experiments place RcsF upstream of RcsC. The RcsF sequence is characteristic of lipoproteins, consistent with a role in sensing cell surface perturbation and transmitting this signal to RcsC. Activation of RcsF does not require increased transcription of the gene, suggesting that modification of the RcsF protein may act as an activating signal. Signals from RcsC require RcsD to activate RcsB. Sequencing of an rcsC allele, rcsC137, that leads to high-level constitutive expression of both cps and rprA suggests that the response regulator domain of RcsC plays a role in negatively regulating the kinase activity of RcsC. The phosphorelay and the variation in the activation mechanism (dependent upon or independent of RcsA) provide multiple steps for modulating the output from this system.

Original languageEnglish (US)
Pages (from-to)6770-6778
Number of pages9
JournalJournal of Bacteriology
Volume187
Issue number19
DOIs
StatePublished - Oct 2005

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Capsules
Escherichia coli
Phosphotransferases
Genes
Lipoproteins
Alleles
RNA
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli. / Majdalani, Nadim; Heck, Michael; Stout, Valerie; Gottesman, Susan.

In: Journal of Bacteriology, Vol. 187, No. 19, 10.2005, p. 6770-6778.

Research output: Contribution to journalArticle

Majdalani, Nadim ; Heck, Michael ; Stout, Valerie ; Gottesman, Susan. / Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli. In: Journal of Bacteriology. 2005 ; Vol. 187, No. 19. pp. 6770-6778.
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