RNA binding properties of conserved protein subunits of human RNase P

Robert Reiner, Noa Alfiya-Mor, Mishka Berrebi-Demma, Donna Wesolowski, Sidney Altman, Nayef Jarrous

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human nuclear RNase P is required for transcription and processing of tRNA. This catalytic RNP has an H1 RNA moiety associated with ten distinct protein subunits. Five (Rpp20, Rpp21, Rpp25, Rpp29 and Pop5) out of eight of these protein subunits, prepared in refolded recombinant forms, bind to H1 RNA in vitro. Rpp20 and Rpp25 bind jointly to H1 RNA, even though each protein can interact independently with this transcript. Nuclease footprinting analysis reveals that Rpp20 and Rpp25 recognize overlapping regions in the P2 and P3 domains of H1 RNA. Rpp21 and Rpp29, which are sufficient for reconstitution of the endonucleolytic activity, bind to separate regions in the catalytic domain of H1 RNA. Common themes and discrepancies in the RNA-protein interactions between human nuclear RNase P and its related yeast and archaeal counterparts provide a rationale for the assembly of the fully active form of this enzyme.

Original languageEnglish (US)
Pages (from-to)5704-5714
Number of pages11
JournalNucleic Acids Research
Volume39
Issue number13
DOIs
StatePublished - Jul 2011
Externally publishedYes

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Ribonuclease P
Protein Subunits
RNA
Transfer RNA
Catalytic Domain
Proteins
Yeasts
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Reiner, R., Alfiya-Mor, N., Berrebi-Demma, M., Wesolowski, D., Altman, S., & Jarrous, N. (2011). RNA binding properties of conserved protein subunits of human RNase P. Nucleic Acids Research, 39(13), 5704-5714. https://doi.org/10.1093/nar/gkr126

RNA binding properties of conserved protein subunits of human RNase P. / Reiner, Robert; Alfiya-Mor, Noa; Berrebi-Demma, Mishka; Wesolowski, Donna; Altman, Sidney; Jarrous, Nayef.

In: Nucleic Acids Research, Vol. 39, No. 13, 07.2011, p. 5704-5714.

Research output: Contribution to journalArticle

Reiner, R, Alfiya-Mor, N, Berrebi-Demma, M, Wesolowski, D, Altman, S & Jarrous, N 2011, 'RNA binding properties of conserved protein subunits of human RNase P', Nucleic Acids Research, vol. 39, no. 13, pp. 5704-5714. https://doi.org/10.1093/nar/gkr126
Reiner R, Alfiya-Mor N, Berrebi-Demma M, Wesolowski D, Altman S, Jarrous N. RNA binding properties of conserved protein subunits of human RNase P. Nucleic Acids Research. 2011 Jul;39(13):5704-5714. https://doi.org/10.1093/nar/gkr126
Reiner, Robert ; Alfiya-Mor, Noa ; Berrebi-Demma, Mishka ; Wesolowski, Donna ; Altman, Sidney ; Jarrous, Nayef. / RNA binding properties of conserved protein subunits of human RNase P. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 13. pp. 5704-5714.
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