Rings are not microtubule assembly intermediates: An analysis of the lag phase in GTP-dependent self-assembly of bovine brain tubulin

Timothy L. Karr, Daniel L. Purich

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The assembly of whole microtubule protein, derived from bovine brain extracts by multiple cycles of temperature-induced assembly and disassembly, exhibits a lag phase in which there is a characteristic, transient drop in the turbidity. This drop is inconsistent with the direct participation of ring-like structures in microtubule assembly, and warming microtubule protein in the presence of GDP unmasks the kinetics of this dissociation phenomenon. These and other data suggest that rings are enthalpy-stabilized and are unlikely intermediates in the entropy-driven self-assembly of microtubules.

Original languageEnglish (US)
Pages (from-to)1885-1889
Number of pages5
JournalTopics in Catalysis
Volume95
Issue number4
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

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