Ribose biosynthesis and evidence for an alternative first step in the common aromatic amino acid pathway in Methanococcus maripaludis

Debra L. Tumbula, Quincy Teng, Michael G. Bartlett, William B. Whitman

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

An acetate-requiring mutant of Methanococcus maripaludis allowed efficient labeling of riboses following growth in minimal medium supplemented with [2-13C]acetate. Nuclear magnetic resonance and mass spectroscopic analysis of purified cytidine and uridine demonstrated that the C-1' of the ribose was about 67% enriched for 13C. This value was inconsistent with the formation of erythrose 4-phosphate (E4P) exclusively by the carboxylation of a triose. Instead, these results suggest that either (i) E4P is formed by both the nonoxidative pentose phosphate and triose carboxylation pathways or (ii) E4P is formed exclusively by the nonoxidative pentose phosphate pathway and is not a precursor of aromatic amino acids.

Original languageEnglish (US)
Pages (from-to)6010-6013
Number of pages4
JournalJournal of bacteriology
Volume179
Issue number19
DOIs
StatePublished - Oct 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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