Ribonucleoside phosphates via phosphorimidazolidate intermediates. Synthesis of pseudoadenosine 5′-triphosphate

John W. Kozarich, A. Craig Chinault, Sidney Hecht

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The use of phosphorimidazolidate intermediates for the conversion of ribonucleoside 5′-monophosphates to diphosphates and 5′-diphosphates to triphosphates is described and several examples are cited. The specific phosphorylation of ribonucleoside 5′-diphosphates to afford the linear rather than branched triphosphates is demonstrated. Also reported is the successful synthesis of the branched isomer of adenosine 5′-triphosphate, φATP, the attempted preparation of which was first reported in 1949. Certain chemical and enzymatic properties of φATP are described, as is the conversion of φATP to ATP via a presumed cyclic metaphosphate intermediate.

Original languageEnglish (US)
Pages (from-to)4458-4463
Number of pages6
JournalBiochemistry
Volume12
Issue number22
StatePublished - 1973
Externally publishedYes

Fingerprint

Ribonucleotides
Diphosphates
Adenosine Triphosphate
Ribonucleosides
Phosphorylation
Isomers
Adenosine
triphosphoric acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ribonucleoside phosphates via phosphorimidazolidate intermediates. Synthesis of pseudoadenosine 5′-triphosphate. / Kozarich, John W.; Chinault, A. Craig; Hecht, Sidney.

In: Biochemistry, Vol. 12, No. 22, 1973, p. 4458-4463.

Research output: Contribution to journalArticle

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