Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa

Effect on cell surface properties and interaction with hydrophobic substrates

R. A. Al-Tahhan, Todd Sandrin, A. A. Bodour, R. M. Maier

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Little is known about the interaction of biosurfactants with bacterial cells. Recent work in the area of biodegradation suggests that there are two mechanisms by which biosurfactants enhance the biodegradation of slightly soluble organic compounds. First, biosurfactants can solubilize hydrophobic compounds within micelle structures, effectively increasing the apparent aqueous solubility of the organic compound and its availability for uptake by a cell. Second, biosurfactants can cause the cell surface to become more hydrophobic, thereby increasing the association of the cell with the slightly soluble substrate. Since the second mechanism requires very low levels of added biosurfactant, it is the more intriguing of the two mechanisms from the perspective of enhancing the biodegradation process. This is because, in practical terms, addition of low levels of biosurfactants will be more cost-effective for bioremediation. To successfully optimize the use of biosurfacrants in the bioremediation process, their effect on cell surfaces must be understood. We report here that rhamnolipid biosurfactant causes the cell surface of Pseudomonas spp. to become hydrophobic through release of lipopolysaccharide (LPS). In this study, two Pseudomonas aeruginosa strains were grown on glucose and hexadecane to investigate the chemical and structural changes that occur in the presence of a rhamnolipid biosurfactant. Results showed that rhamnolipids caused an overall loss in cellular fatty acid content. Loss of fatty acids was due to release of LPS from the outer membrane, as demonstrated by 2-keto-3-deoxyoctonic acid and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and further confirmed by scanning electron microscopy. The amount of LPS loss was found to be dependent on rhamnolipid concentration, but significant loss occurred even at concentrations less than the critical micelle concentration. We conclude that rhamnolipid-induced LPS release is the probable mechanism of enhanced cell surface hydrophobicity.

Original languageEnglish (US)
Pages (from-to)3262-3268
Number of pages7
JournalApplied and Environmental Microbiology
Volume66
Issue number8
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

biosurfactants
Surface Properties
Hydrophobic and Hydrophilic Interactions
Pseudomonas aeruginosa
lipopolysaccharides
Lipopolysaccharides
substrate
biodegradation
bioremediation
organic compound
fatty acid
cells
Environmental Biodegradation
Micelles
micelles
hydrophobicity
organic compounds
structural change
Fatty Acids
electrokinesis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa : Effect on cell surface properties and interaction with hydrophobic substrates. / Al-Tahhan, R. A.; Sandrin, Todd; Bodour, A. A.; Maier, R. M.

In: Applied and Environmental Microbiology, Vol. 66, No. 8, 2000, p. 3262-3268.

Research output: Contribution to journalArticle

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