TY - JOUR
T1 - Rewiring photosynthesis
T2 - A photosystem I-hydrogenase chimera that makes H2: In vivo
AU - Kanygin, Andrey
AU - Milrad, Yuval
AU - Thummala, Chandrasekhar
AU - Reifschneider, Kiera
AU - Baker, Patricia
AU - Marco, Pini
AU - Yacoby, Iftach
AU - Redding, Kevin E.
N1 - Funding Information:
This material is based upon work supported by the National Science Foundation under Grant No. CBET-1706960. The Tel Aviv University group was supported by the Israel Science Foundation award 1646/16, the NSF-BSF Energy for Sustainability grant 2016666, and Israel Science Foundation award 2185/17.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2020/9
Y1 - 2020/9
N2 - Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As a proof of concept, an in vivo fusion of photosystem I (PSI) and algal hydrogenase was created by insertion of the HydA sequence into the PsaC subunit. The PSI and hydrogenase portions are co-assembled and active in vivo, effectively creating a new photosystem. Cells expressing only the PSI-hydrogenase chimera make hydrogen at high rates in a light-dependent fashion for several days. In these engineered cells, photosynthetic electron flow is directed away from CO2 fixation and towards proton reduction, demonstrating the possibility of driving novel redox chemistries using electrons from water splitting and the photosynthetic electron transport chain. This journal is
AB - Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As a proof of concept, an in vivo fusion of photosystem I (PSI) and algal hydrogenase was created by insertion of the HydA sequence into the PsaC subunit. The PSI and hydrogenase portions are co-assembled and active in vivo, effectively creating a new photosystem. Cells expressing only the PSI-hydrogenase chimera make hydrogen at high rates in a light-dependent fashion for several days. In these engineered cells, photosynthetic electron flow is directed away from CO2 fixation and towards proton reduction, demonstrating the possibility of driving novel redox chemistries using electrons from water splitting and the photosynthetic electron transport chain. This journal is
UR - http://www.scopus.com/inward/record.url?scp=85092437506&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092437506&partnerID=8YFLogxK
U2 - 10.1039/c9ee03859k
DO - 10.1039/c9ee03859k
M3 - Article
AN - SCOPUS:85092437506
SN - 1754-5692
VL - 13
SP - 2903
EP - 2914
JO - Energy and Environmental Science
JF - Energy and Environmental Science
IS - 9
ER -