Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor

E. Bücking-Throm, W. Duntze, L. H. Hartwell, T. R. Manney

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193 Scopus citations


A diffusible substance, α factor, is produced constitutively by haploid yeast cells of α mating type and this factor specifically inhibits the division of a mating type cells. Experiments are presented which demonstrate that α factor arrests a cells as unbudded, mononucleate cells prior to the initiation of DNA synthesis in the cell cycle. Studies with temperature-sensitive mutants defective in one of thirteen different cell cycle functions suggest that although arrested a cells continue to enlarge they do not perform functions required for the next cell cycle. The arrest is reversible and a partially synchronized round of DNA replication is observed upon removal of α factor from arrested cells. We propose that this factor is one element of a regulatory system that functions to assure the synchronization of a and α haploid cell cycles prior to conjugation.

Original languageEnglish (US)
Pages (from-to)99-110
Number of pages12
JournalExperimental Cell Research
Issue number1
StatePublished - Jan 1973
Externally publishedYes


ASJC Scopus subject areas

  • Cell Biology

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