Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor

E. Bücking-Throm, W. Duntze, Leland Hartwell, T. R. Manney

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

A diffusible substance, α factor, is produced constitutively by haploid yeast cells of α mating type and this factor specifically inhibits the division of a mating type cells. Experiments are presented which demonstrate that α factor arrests a cells as unbudded, mononucleate cells prior to the initiation of DNA synthesis in the cell cycle. Studies with temperature-sensitive mutants defective in one of thirteen different cell cycle functions suggest that although arrested a cells continue to enlarge they do not perform functions required for the next cell cycle. The arrest is reversible and a partially synchronized round of DNA replication is observed upon removal of α factor from arrested cells. We propose that this factor is one element of a regulatory system that functions to assure the synchronization of a and α haploid cell cycles prior to conjugation.

Original languageEnglish (US)
Pages (from-to)99-110
Number of pages12
JournalExperimental Cell Research
Volume76
Issue number1
DOIs
StatePublished - 1973
Externally publishedYes

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Sex Factors
Haploidy
Yeasts
Cell Cycle
DNA
Mating Factor
DNA Replication
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor. / Bücking-Throm, E.; Duntze, W.; Hartwell, Leland; Manney, T. R.

In: Experimental Cell Research, Vol. 76, No. 1, 1973, p. 99-110.

Research output: Contribution to journalArticle

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