Abstract
A diffusible substance, α factor, is produced constitutively by haploid yeast cells of α mating type and this factor specifically inhibits the division of a mating type cells. Experiments are presented which demonstrate that α factor arrests a cells as unbudded, mononucleate cells prior to the initiation of DNA synthesis in the cell cycle. Studies with temperature-sensitive mutants defective in one of thirteen different cell cycle functions suggest that although arrested a cells continue to enlarge they do not perform functions required for the next cell cycle. The arrest is reversible and a partially synchronized round of DNA replication is observed upon removal of α factor from arrested cells. We propose that this factor is one element of a regulatory system that functions to assure the synchronization of a and α haploid cell cycles prior to conjugation.
Original language | English (US) |
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Pages (from-to) | 99-110 |
Number of pages | 12 |
Journal | Experimental Cell Research |
Volume | 76 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1973 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology