Retinol-binding protein 4 is associated with impaired glucose tolerance but not with whole body or hepatic insulin resistance in Mexican Americans

Alberto O. Chavez, Dawn K. Coletta, Subhash Kamath, Douglas T. Cromack, Adriana Monroy, Franco Folli, Ralph A. DeFronzo, Devjit Tripathy

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Retinol-binding protein-4 (RBP4), a novel protein secreted mainly by adipose tissue, has been associated with insulin resistance in obese subjects and in individuals with type 2 diabetes mellitus (T2DM). We examined the relationship between plasma RBP4 levels, expression of RBP4 in skeletal muscle and adipose tissue, and insulin sensitivity in Mexican Americans with varying degrees of obesity and glucose tolerance. Seventy-two subjects [16 lean normal-glucose-tolerant (NGT), 17 obese NGT, and 39 subjects with impaired fasting glucose/impaired glucose tolerance/T2DM] received an oral glucose tolerance test (OGTT) and euglycemic-hyperinsulinemic clamp. Insulin secretion was measured as insulinogenic index during OGTT. In a subset of subjects, hepatic glucose production was measured by 3-[3H]glucose infusion, biopsies of the vastus lateralis muscle and subcutaneous adipose tissue were obtained under basal conditions, and quantitative RT-PCR was performed to measure the RBP4 mRNA gene expression. Plasma RBP4 was significantly elevated in impaired glucose tolerance/T2DM compared with NGT lean or obese subjects. Plasma RBP4 levels correlated with 2-h glucose, triglycerides, and hemoglobin A1c. There was no association between RBP4 levels and whole body insulin sensitivity measured with either the euglycemic insulin clamp or OGTT, basal hepatic glucose production rates, and the hepatic insulin resistance index. There was no correlation between plasma RBP4 levels and indexes of insulin secretion. RBP4 mRNA expression in skeletal muscle was similar in lean NGT subjects, obese NGT subjects, and T2DM subjects. There was no difference in RBP4 mRNA expression in adipose tissue between lean and obese NGT subjects or between NGT and T2DM individuals. Plasma RBP4 levels are elevated in T2DM and associated with impaired glucose tolerance, but not associated with obesity or insulin resistance or impaired insulin secretion in Mexican Americans.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume296
Issue number4
DOIs
StatePublished - Apr 2009
Externally publishedYes

Fingerprint

Retinol-Binding Proteins
Glucose Intolerance
Insulin Resistance
Glucose
Plasma Retinol-Binding Proteins
Liver
Type 2 Diabetes Mellitus
Glucose Tolerance Test
Insulin
Adipose Tissue
Glucose Clamp Technique
Messenger RNA
Skeletal Muscle
Obesity
Muscles
Subcutaneous Fat
Quadriceps Muscle
Fasting

Keywords

  • Adipokines
  • Insulin sensitivity
  • Pathogenesis
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Retinol-binding protein 4 is associated with impaired glucose tolerance but not with whole body or hepatic insulin resistance in Mexican Americans. / Chavez, Alberto O.; Coletta, Dawn K.; Kamath, Subhash; Cromack, Douglas T.; Monroy, Adriana; Folli, Franco; DeFronzo, Ralph A.; Tripathy, Devjit.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 296, No. 4, 04.2009.

Research output: Contribution to journalArticle

Chavez, Alberto O. ; Coletta, Dawn K. ; Kamath, Subhash ; Cromack, Douglas T. ; Monroy, Adriana ; Folli, Franco ; DeFronzo, Ralph A. ; Tripathy, Devjit. / Retinol-binding protein 4 is associated with impaired glucose tolerance but not with whole body or hepatic insulin resistance in Mexican Americans. In: American Journal of Physiology - Endocrinology and Metabolism. 2009 ; Vol. 296, No. 4.
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AU - Cromack, Douglas T.

AU - Monroy, Adriana

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AU - DeFronzo, Ralph A.

AU - Tripathy, Devjit

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