Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

Bradley Doebbeling, M. A. Pfaller, R. J. Hollis, L. D. Boyken, A. C. Pignatari, L. A. Herwaldt, R. P. Wenzel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

Original languageEnglish (US)
Pages (from-to)4-8
Number of pages5
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Volume11
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

Fingerprint

DNA Restriction Enzymes
Staphylococcus aureus
Plasmids
DNA
Molecular Typing
Brazil

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

Cite this

Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents. / Doebbeling, Bradley; Pfaller, M. A.; Hollis, R. J.; Boyken, L. D.; Pignatari, A. C.; Herwaldt, L. A.; Wenzel, R. P.

In: European Journal of Clinical Microbiology & Infectious Diseases, Vol. 11, No. 1, 01.1992, p. 4-8.

Research output: Contribution to journalArticle

Doebbeling, Bradley ; Pfaller, M. A. ; Hollis, R. J. ; Boyken, L. D. ; Pignatari, A. C. ; Herwaldt, L. A. ; Wenzel, R. P. / Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents. In: European Journal of Clinical Microbiology & Infectious Diseases. 1992 ; Vol. 11, No. 1. pp. 4-8.
@article{2606a3c5f6194c938b6fd0db0aa13ccb,
title = "Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents",
abstract = "Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 {\%} of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 {\%} on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.",
author = "Bradley Doebbeling and Pfaller, {M. A.} and Hollis, {R. J.} and Boyken, {L. D.} and Pignatari, {A. C.} and Herwaldt, {L. A.} and Wenzel, {R. P.}",
year = "1992",
month = "1",
doi = "10.1007/BF01971263",
language = "English (US)",
volume = "11",
pages = "4--8",
journal = "European Journal of Clinical Microbiology and Infectious Diseases",
issn = "0934-9723",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

AU - Doebbeling, Bradley

AU - Pfaller, M. A.

AU - Hollis, R. J.

AU - Boyken, L. D.

AU - Pignatari, A. C.

AU - Herwaldt, L. A.

AU - Wenzel, R. P.

PY - 1992/1

Y1 - 1992/1

N2 - Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

AB - Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

UR - http://www.scopus.com/inward/record.url?scp=0026526052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026526052&partnerID=8YFLogxK

U2 - 10.1007/BF01971263

DO - 10.1007/BF01971263

M3 - Article

C2 - 1563382

AN - SCOPUS:0026526052

VL - 11

SP - 4

EP - 8

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

IS - 1

ER -