Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

B. N. Doebbeling; M. A. Pfaller; R. J. Hollis; L. D. Boyken; A. C. Pignatari; L. A. Herwaldt; R. P. Wenzel

doi:10.1007/BF01971263

Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

B. N. Doebbeling, M. A. Pfaller, R. J. Hollis, L. D. Boyken, A. C. Pignatari, L. A. Herwaldt, R. P. Wenzel

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18 Scopus citations

Abstract

Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

Original language	English (US)
Pages (from-to)	4-8
Number of pages	5
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/BF01971263
State	Published - Jan 1992
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1007/BF01971263

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title = "Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents",

abstract = "Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.",

author = "Doebbeling, {B. N.} and Pfaller, {M. A.} and Hollis, {R. J.} and Boyken, {L. D.} and Pignatari, {A. C.} and Herwaldt, {L. A.} and Wenzel, {R. P.}",

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AU - Doebbeling, B. N.

AU - Pfaller, M. A.

AU - Hollis, R. J.

AU - Boyken, L. D.

AU - Pignatari, A. C.

AU - Herwaldt, L. A.

AU - Wenzel, R. P.

PY - 1992/1

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AB - Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

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Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

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