Abstract

This study evaluated how Synechocystis sp. PCC 6803 responds to high total dissolved solids (TDS) associated with eliminating nutrient limitation during long-term operation of a photobioreactor. The unique feature is that the TDS were not dominated by Na+ and Cl-, as in seawater, but by HCO3 - and NO3 - from nutrient delivery. The TDS-stress threshold was about 10g/L. Whereas inorganic N and P limitations slowed the rate of inorganic C (Ci) uptake in the light, TDS stress was manifested most strongly as a substantial increase of endogenous respiration rate at night. Relief from TDS stress was incomplete when lowered pH led to a HCO3 - increase (560mgC/L as a threshold). Impaired photosynthesis led to a cascade of reduced Ci-uptake, pH decrease, HCO3 - accumulation, and HCO3 --associated stress. Thus, long-term photobioreactor operation requires balancing the delivery rates of CO2, N, P, and other TDS components to avoid general and Ci-associated TDS stresses.

Original languageEnglish (US)
Pages (from-to)378-384
Number of pages7
JournalBioresource Technology
Volume128
DOIs
StatePublished - Jan 2013

Fingerprint

Photobioreactors
Nutrients
Photosynthesis
nutrient limitation
Seawater
photosynthesis
respiration
relief
seawater
nutrient

Keywords

  • Inorganic carbon
  • Stress
  • Synechocystis
  • Total dissolved solids

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Responses of synechocystis sp. PCC 6803 to total dissolved solids in long-term continuous operation of a photobioreactor. / Kim, Hyun Woo; Vannela, Raveender; Rittmann, Bruce.

In: Bioresource Technology, Vol. 128, 01.2013, p. 378-384.

Research output: Contribution to journalArticle

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