Response thresholds in bacterial chemotaxis

Pushkar P. Lele, Abhishek Shrivastava, Thibault Roland, Howard C. Berg

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Stimulation of Escherichia coli by exponential ramps of chemoattractants generates step changes in the concentration of the response regulator, CheY-P. Because flagellar motors are ultrasensitive, this should change the fraction of time that motors spin clockwise, the CWbias. However, early work failed to show changes in CWbias when ramps were shallow. This was explained by a model for motor remodeling that predicted plateaus in plots of CWbias versus [CheY-P]. We looked for these plateaus by examining distributions of CWbias in populations of cells with different mean [CheY-P]. We did not find such plateaus. Hence, we repeated the work on shallow ramps and found that motors did indeed respond. These responses were quantitatively described by combining motor remodeling with ultrasensitivity in a model that exhibited high sensitivities over a wide dynamic range.

Original languageEnglish (US)
Article number1500299
JournalScience Advances
Volume1
Issue number9
DOIs
StatePublished - Oct 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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