Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes

Ximei Zhang, Wei Liu, Michael Schloter, Guangming Zhang, Quansheng Chen, Jianhui Huang, Linghao Li, James Elser, Xingguo Han

Research output: Contribution to journalArticle

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Abstract

Multiple co-occurring environmental changes are affecting soil nitrogen cycling processes, which are mainly mediated by microbes. While it is likely that various nitrogen-cycling functional groups will respond differently to such environmental changes, very little is known about their relative responsiveness. Here we conducted four long-term experiments in a steppe ecosystem by removing plant functional groups, mowing, adding nitrogen, adding phosphorus, watering, warming, and manipulating some of their combinations. We quantified the abundance of seven nitrogen-cycling genes, including those for fixation (nifH), mineralization (chiA), nitrification (amoA of ammonia-oxidizing bacteria (AOB) or archaea (AOA)), and denitrification (nirS, nirK and nosZ). First, for each gene, we compared its sensitivities to different environmental changes and found that the abundances of various genes were sensitive to distinct and different factors. Overall, the abundances of nearly all genes were sensitive to nitrogen enrichment. In addition, the abundances of the chiA and nosZ genes were sensitive to plant functional group removal, the AOB-amoA gene abundance to phosphorus enrichment when nitrogen was added simultaneously, and the nirS and nirK gene abundances responded to watering. Second, for each single- or multi-factorial environmental change, we compared the sensitivities of the abundances of different genes and found that different environmental changes primarily affected different gene abundances. Overall, AOB-amoA gene abundance was most responsive, followed by the two denitrifying genes nosZ and nirS, while the other genes were less sensitive. These results provide, for the first time, systematic insights into how the abundance of each type of nitrogen-cycling gene and the equilibrium state of all these nitrogen-cycling gene abundances would shift under each single- or multi-factorial global change.

Original languageEnglish (US)
Article numbere76500
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 4 2013

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global change
Nitrogen
Soil
Genes
Soils
nitrogen
soil
genes
Ammonia
Functional groups
Bacteria
ammonia
Phosphorus
bacteria
irrigation
Nitrogen fixation
Nitrification
Denitrification
phosphorus
Archaea

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Zhang, X., Liu, W., Schloter, M., Zhang, G., Chen, Q., Huang, J., ... Han, X. (2013). Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes. PLoS One, 8(10), [e76500]. https://doi.org/10.1371/journal.pone.0076500

Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes. / Zhang, Ximei; Liu, Wei; Schloter, Michael; Zhang, Guangming; Chen, Quansheng; Huang, Jianhui; Li, Linghao; Elser, James; Han, Xingguo.

In: PLoS One, Vol. 8, No. 10, e76500, 04.10.2013.

Research output: Contribution to journalArticle

Zhang, X, Liu, W, Schloter, M, Zhang, G, Chen, Q, Huang, J, Li, L, Elser, J & Han, X 2013, 'Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes', PLoS One, vol. 8, no. 10, e76500. https://doi.org/10.1371/journal.pone.0076500
Zhang, Ximei ; Liu, Wei ; Schloter, Michael ; Zhang, Guangming ; Chen, Quansheng ; Huang, Jianhui ; Li, Linghao ; Elser, James ; Han, Xingguo. / Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes. In: PLoS One. 2013 ; Vol. 8, No. 10.
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