Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells

G. Poste; B. Schaeffer; P. Reeve; D. J. Alexander

doi:10.1016/0042-6822(74)90368-7

Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells

G. Poste, B. Schaeffer, P. Reeve, D. J. Alexander

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The formation of heterokaryons by fusion of anucleate African green monkey kidney cells with simian virus 40 (SV40)-transformed mouse 3T3 (SV3T3) cells resulted in the rescue of infective SV40. The yield of SV40 rescued from the heterokaryons was influenced by the physiological state of the monkey cells. Fusion of SV3T3 cells with monkey cells from actively dividing subconfluent log phase cultures resulted in the rescue of significantly lower yields of SV40 than from heterokaryons produced between SV3T3 cells and monkey cells from nondividing confluent stationary phase cultures. This effect was found with both anucleate and nucleated monkey cells.

Original language	English (US)
Pages (from-to)	85-95
Number of pages	11
Journal	Virology
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(74)90368-7
State	Published - Jul 1974
Externally published	Yes

ASJC Scopus subject areas

Virology

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@article{3763fe69b7254ba084cacd5e2592da75,

title = "Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells",

abstract = "The formation of heterokaryons by fusion of anucleate African green monkey kidney cells with simian virus 40 (SV40)-transformed mouse 3T3 (SV3T3) cells resulted in the rescue of infective SV40. The yield of SV40 rescued from the heterokaryons was influenced by the physiological state of the monkey cells. Fusion of SV3T3 cells with monkey cells from actively dividing subconfluent log phase cultures resulted in the rescue of significantly lower yields of SV40 than from heterokaryons produced between SV3T3 cells and monkey cells from nondividing confluent stationary phase cultures. This effect was found with both anucleate and nucleated monkey cells.",

author = "G. Poste and B. Schaeffer and P. Reeve and Alexander, {D. J.}",

note = "Funding Information: This work was supported by Grant lROlCA-13393 from the National Institutes of Health. We thank Joe Sambrook for his helpful advice. The technical assistance of P. Newhouse, K. Willett, and A. MacKearnin is gratefully acknowledged.",

year = "1974",

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doi = "10.1016/0042-6822(74)90368-7",

language = "English (US)",

volume = "60",

pages = "85--95",

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T1 - Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells

AU - Poste, G.

AU - Schaeffer, B.

AU - Reeve, P.

AU - Alexander, D. J.

N1 - Funding Information: This work was supported by Grant lROlCA-13393 from the National Institutes of Health. We thank Joe Sambrook for his helpful advice. The technical assistance of P. Newhouse, K. Willett, and A. MacKearnin is gratefully acknowledged.

PY - 1974/7

Y1 - 1974/7

N2 - The formation of heterokaryons by fusion of anucleate African green monkey kidney cells with simian virus 40 (SV40)-transformed mouse 3T3 (SV3T3) cells resulted in the rescue of infective SV40. The yield of SV40 rescued from the heterokaryons was influenced by the physiological state of the monkey cells. Fusion of SV3T3 cells with monkey cells from actively dividing subconfluent log phase cultures resulted in the rescue of significantly lower yields of SV40 than from heterokaryons produced between SV3T3 cells and monkey cells from nondividing confluent stationary phase cultures. This effect was found with both anucleate and nucleated monkey cells.

AB - The formation of heterokaryons by fusion of anucleate African green monkey kidney cells with simian virus 40 (SV40)-transformed mouse 3T3 (SV3T3) cells resulted in the rescue of infective SV40. The yield of SV40 rescued from the heterokaryons was influenced by the physiological state of the monkey cells. Fusion of SV3T3 cells with monkey cells from actively dividing subconfluent log phase cultures resulted in the rescue of significantly lower yields of SV40 than from heterokaryons produced between SV3T3 cells and monkey cells from nondividing confluent stationary phase cultures. This effect was found with both anucleate and nucleated monkey cells.

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Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells

Abstract

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