Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells

G. Poste, B. Schaeffer, P. Reeve, D. J. Alexander

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The formation of heterokaryons by fusion of anucleate African green monkey kidney cells with simian virus 40 (SV40)-transformed mouse 3T3 (SV3T3) cells resulted in the rescue of infective SV40. The yield of SV40 rescued from the heterokaryons was influenced by the physiological state of the monkey cells. Fusion of SV3T3 cells with monkey cells from actively dividing subconfluent log phase cultures resulted in the rescue of significantly lower yields of SV40 than from heterokaryons produced between SV3T3 cells and monkey cells from nondividing confluent stationary phase cultures. This effect was found with both anucleate and nucleated monkey cells.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalVirology
Volume60
Issue number1
DOIs
StatePublished - Jul 1974
Externally publishedYes

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Rescue of simian virus 40 (SV40) from SV40-transformed cells by fusion with anucleate monkey cells and variation in the yield of virus rescued by fusion with replicating or nonreplicating monkey cells'. Together they form a unique fingerprint.

Cite this