Requirement of phospholipase D1 activity in H-RasV12-induced transformation

F. Gregory Buchanan, Matt McReynolds, Anthony Couvillon, Yoonseok Kam, Vijaykumar R. Holla, Raymond N. DuBois, John H. Exton

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The ability of the Ras oncogene to transform normal cells has been well established. One downstream effector of Ras is the lipid hydrolyzing enzyme phospholipase D. Recent evidence has emerged indicating a role for phospholipase D in cell proliferation, membrane trafficking, and migration. To study the potential importance of phospholipase D in the oncogenic ability of Ras, we used Rat-2 fibroblasts with reduced phospholipase D1 activity (Rat-2V25). Here, we show that H-Ras transformation of Rat-2 fibroblasts requires normal phospholipase D1 activity. WT Rat-2 fibroblasts transfected with the H-Ras V12 oncogene grew colonies in soft agar and tumors in nude mice. However, Rat-2V25 cells when transfected with the H-RasV12 oncogene did not form colonies in soft agar or produce tumors when xenografted onto nude mice. Interestingly, in the presence of phosphatidic acid, the product of phospholipase D, growth in soft agar and tumor formation was restored. We also observed a dramatic increase in the expression of phospholipase D1 in colorectal tumors when compared with adjacent normal mucosa. Our studies identify phospholipase D1 as a critical downstream mediator of H-Ras-induced tumor formation.

Original languageEnglish (US)
Pages (from-to)1638-1642
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number5
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

Fingerprint

Phospholipase D
Agar
ras Genes
Fibroblasts
Nude Mice
Neoplasms
Phosphatidic Acids
Oncogenes
Colorectal Neoplasms
Mucous Membrane
Cell Proliferation
Cell Membrane
Lipids
phospholipase D1
Enzymes
Growth

Keywords

  • Colorectal
  • Phosphatidic acid
  • Ras
  • Xenograft

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Buchanan, F. G., McReynolds, M., Couvillon, A., Kam, Y., Holla, V. R., DuBois, R. N., & Exton, J. H. (2005). Requirement of phospholipase D1 activity in H-RasV12-induced transformation. Proceedings of the National Academy of Sciences of the United States of America, 102(5), 1638-1642. https://doi.org/10.1073/pnas.0406698102

Requirement of phospholipase D1 activity in H-RasV12-induced transformation. / Buchanan, F. Gregory; McReynolds, Matt; Couvillon, Anthony; Kam, Yoonseok; Holla, Vijaykumar R.; DuBois, Raymond N.; Exton, John H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 5, 01.02.2005, p. 1638-1642.

Research output: Contribution to journalArticle

Buchanan, FG, McReynolds, M, Couvillon, A, Kam, Y, Holla, VR, DuBois, RN & Exton, JH 2005, 'Requirement of phospholipase D1 activity in H-RasV12-induced transformation', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 5, pp. 1638-1642. https://doi.org/10.1073/pnas.0406698102
Buchanan, F. Gregory ; McReynolds, Matt ; Couvillon, Anthony ; Kam, Yoonseok ; Holla, Vijaykumar R. ; DuBois, Raymond N. ; Exton, John H. / Requirement of phospholipase D1 activity in H-RasV12-induced transformation. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 5. pp. 1638-1642.
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