Reproducibility of measurement techniques used for creatine kinase, interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females

Bryna Chrismas, Lee Taylor, Alexander Smith, Philip Pemberton, Jason Charles Siegler, Adrian Wayne Midgley

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

To examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were −69.7 to 63.5 IU/L for CK, −1.48 to 1.80 pg/ml for IL-6 and −1.10 to 0.91 µg/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect.

Original languageEnglish (US)
Pages (from-to)191-199
Number of pages9
JournalMeasurement in Physical Education and Exercise Science
Volume22
Issue number3
DOIs
StatePublished - Jul 3 2018
Externally publishedYes

Keywords

  • biomarkers
  • cytokines
  • Exercise induced muscle damage
  • inflammation
  • reliability

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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