Report on maize streak virus in the South African sugar industry

T. Van Antwerpen, S. A. McFarlane, P. Govender, B. A M Potier, M. J. Way, B. Flett, M. Ramusi, Arvind Varsani, D. N. Shepherd, M. Stiller, D. P. Martin, T. M. Webster

Research output: Contribution to journalArticle

Abstract

Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).

Original languageEnglish (US)
Pages (from-to)348-354
Number of pages7
JournalInternational Sugar Journal
Volume116
Issue number1385
StatePublished - May 1 2014
Externally publishedYes

Fingerprint

Maize streak virus
sugar industry
Cicadulina mbila
Industry
Cicadellidae
Hemiptera
Saccharum
Exitianus
Balclutha incisa
signs and symptoms (plants)
Balclutha
Cicadulina
coasts
Electron Transport Complex IV
Virus Diseases
cytochrome-c oxidase
Zea mays
sugarcane
Viruses
genomics

Keywords

  • Cicadulina spp.
  • Disease identification
  • Insect classification
  • Leafhoppers
  • Maize Streak Virus
  • Sugarcane
  • Sugarcane diseases

ASJC Scopus subject areas

  • Food Science

Cite this

Van Antwerpen, T., McFarlane, S. A., Govender, P., Potier, B. A. M., Way, M. J., Flett, B., ... Webster, T. M. (2014). Report on maize streak virus in the South African sugar industry. International Sugar Journal, 116(1385), 348-354.

Report on maize streak virus in the South African sugar industry. / Van Antwerpen, T.; McFarlane, S. A.; Govender, P.; Potier, B. A M; Way, M. J.; Flett, B.; Ramusi, M.; Varsani, Arvind; Shepherd, D. N.; Stiller, M.; Martin, D. P.; Webster, T. M.

In: International Sugar Journal, Vol. 116, No. 1385, 01.05.2014, p. 348-354.

Research output: Contribution to journalArticle

Van Antwerpen, T, McFarlane, SA, Govender, P, Potier, BAM, Way, MJ, Flett, B, Ramusi, M, Varsani, A, Shepherd, DN, Stiller, M, Martin, DP & Webster, TM 2014, 'Report on maize streak virus in the South African sugar industry', International Sugar Journal, vol. 116, no. 1385, pp. 348-354.
Van Antwerpen T, McFarlane SA, Govender P, Potier BAM, Way MJ, Flett B et al. Report on maize streak virus in the South African sugar industry. International Sugar Journal. 2014 May 1;116(1385):348-354.
Van Antwerpen, T. ; McFarlane, S. A. ; Govender, P. ; Potier, B. A M ; Way, M. J. ; Flett, B. ; Ramusi, M. ; Varsani, Arvind ; Shepherd, D. N. ; Stiller, M. ; Martin, D. P. ; Webster, T. M. / Report on maize streak virus in the South African sugar industry. In: International Sugar Journal. 2014 ; Vol. 116, No. 1385. pp. 348-354.
@article{356d48bac9a54385bf4b84afdcf6e68b,
title = "Report on maize streak virus in the South African sugar industry",
abstract = "Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31{\%} in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naud{\'e}), Cicadulina anestae van Rensburg and C. mbila(Naud{\'e}).",
keywords = "Cicadulina spp., Disease identification, Insect classification, Leafhoppers, Maize Streak Virus, Sugarcane, Sugarcane diseases",
author = "{Van Antwerpen}, T. and McFarlane, {S. A.} and P. Govender and Potier, {B. A M} and Way, {M. J.} and B. Flett and M. Ramusi and Arvind Varsani and Shepherd, {D. N.} and M. Stiller and Martin, {D. P.} and Webster, {T. M.}",
year = "2014",
month = "5",
day = "1",
language = "English (US)",
volume = "116",
pages = "348--354",
journal = "International Sugar Journal",
issn = "0020-8841",
publisher = "Agra Europe Ltd.",
number = "1385",

}

TY - JOUR

T1 - Report on maize streak virus in the South African sugar industry

AU - Van Antwerpen, T.

AU - McFarlane, S. A.

AU - Govender, P.

AU - Potier, B. A M

AU - Way, M. J.

AU - Flett, B.

AU - Ramusi, M.

AU - Varsani, Arvind

AU - Shepherd, D. N.

AU - Stiller, M.

AU - Martin, D. P.

AU - Webster, T. M.

PY - 2014/5/1

Y1 - 2014/5/1

N2 - Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).

AB - Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).

KW - Cicadulina spp.

KW - Disease identification

KW - Insect classification

KW - Leafhoppers

KW - Maize Streak Virus

KW - Sugarcane

KW - Sugarcane diseases

UR - http://www.scopus.com/inward/record.url?scp=84916600729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916600729&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84916600729

VL - 116

SP - 348

EP - 354

JO - International Sugar Journal

JF - International Sugar Journal

SN - 0020-8841

IS - 1385

ER -