Abstract
Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 348-354 |
| Number of pages | 7 |
| Journal | International Sugar Journal |
| Volume | 116 |
| Issue number | 1385 |
| State | Published - May 1 2014 |
| Externally published | Yes |
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Keywords
- Cicadulina spp.
- Disease identification
- Insect classification
- Leafhoppers
- Maize Streak Virus
- Sugarcane
- Sugarcane diseases
ASJC Scopus subject areas
- Food Science
Cite this
Report on maize streak virus in the South African sugar industry. / Van Antwerpen, T.; McFarlane, S. A.; Govender, P.; Potier, B. A M; Way, M. J.; Flett, B.; Ramusi, M.; Varsani, Arvind; Shepherd, D. N.; Stiller, M.; Martin, D. P.; Webster, T. M.
In: International Sugar Journal, Vol. 116, No. 1385, 01.05.2014, p. 348-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Report on maize streak virus in the South African sugar industry
AU - Van Antwerpen, T.
AU - McFarlane, S. A.
AU - Govender, P.
AU - Potier, B. A M
AU - Way, M. J.
AU - Flett, B.
AU - Ramusi, M.
AU - Varsani, Arvind
AU - Shepherd, D. N.
AU - Stiller, M.
AU - Martin, D. P.
AU - Webster, T. M.
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).
AB - Symptoms caused by Maize Streak Virus (MSV) were first observed in the Lower South Coast region of the South African sugarcane industry in 2007 on variety N44. Identical symptoms were subsequently observed on varieties N44 and N36 in the Midlands North and South Coast areas and on N36 in Mpumalanga. Phylogenetic analysis determined that the virus closely resembled the South African maize-adapted MSV strain, MSV-A, subtype MSV-A4. In a pot trial MSV infection resulted in an average yield loss of 31% in infected N44 plants. A colony of Cicadulina mbila was established at SASRI to screen commercial sugarcane cultivars for their susceptibility to MSV Cytochrome oxidase C subunit primers were used to sequence genomic DNA from C. mbila for molecular identification of the leafhopper. A PCR protocol was established for the detection of MSV in the leafhopper C. mbila. The diversity and distribution of leafhopper species in the sugarcane industry is currently being monitored. Thirteen species of leafhoppers (Cicadellidae) were collected during the first season of monitoring: Gambialoa sp., Afrosteles sp., Aconurella prolixa (Lethierry), Exitianus frontalis (Distant), Exitianus taeniaticeps (Kirchbaum), Empoascanara ethiopica Dworakowska, Molopopterus spp., Maiestas dolabra (Kramer), Balclutha rosea Scott, Balclutha incisa Matsumura, Afrosus unimaculatus (Naudé), Cicadulina anestae van Rensburg and C. mbila(Naudé).
KW - Cicadulina spp.
KW - Disease identification
KW - Insect classification
KW - Leafhoppers
KW - Maize Streak Virus
KW - Sugarcane
KW - Sugarcane diseases
UR - http://www.scopus.com/inward/record.url?scp=84916600729&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84916600729&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84916600729
VL - 116
SP - 348
EP - 354
JO - International Sugar Journal
JF - International Sugar Journal
SN - 0020-8841
IS - 1385
ER -