Replicative intermediates of maize streak virus found during leaf development

Julia B. Erdmann, Dionne N. Shepherd, Darren P. Martin, Arvind Varsani, Edward P. Rybicki, Holger Jeske

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR.

Original languageEnglish (US)
Pages (from-to)1077-1081
Number of pages5
JournalJournal of General Virology
Volume91
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

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Maize streak virus
Geminiviridae
Begomovirus
Circular DNA
Electrophoresis, Gel, Two-Dimensional
Regulator Genes
DNA Replication
Zea mays

ASJC Scopus subject areas

  • Virology

Cite this

Erdmann, J. B., Shepherd, D. N., Martin, D. P., Varsani, A., Rybicki, E. P., & Jeske, H. (2010). Replicative intermediates of maize streak virus found during leaf development. Journal of General Virology, 91(4), 1077-1081. https://doi.org/10.1099/vir.0.017574-0

Replicative intermediates of maize streak virus found during leaf development. / Erdmann, Julia B.; Shepherd, Dionne N.; Martin, Darren P.; Varsani, Arvind; Rybicki, Edward P.; Jeske, Holger.

In: Journal of General Virology, Vol. 91, No. 4, 04.2010, p. 1077-1081.

Research output: Contribution to journalArticle

Erdmann, JB, Shepherd, DN, Martin, DP, Varsani, A, Rybicki, EP & Jeske, H 2010, 'Replicative intermediates of maize streak virus found during leaf development', Journal of General Virology, vol. 91, no. 4, pp. 1077-1081. https://doi.org/10.1099/vir.0.017574-0
Erdmann, Julia B. ; Shepherd, Dionne N. ; Martin, Darren P. ; Varsani, Arvind ; Rybicki, Edward P. ; Jeske, Holger. / Replicative intermediates of maize streak virus found during leaf development. In: Journal of General Virology. 2010 ; Vol. 91, No. 4. pp. 1077-1081.
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